Protein interactions and misfolding analyzed by AFM force spectroscopy

Chad McAllister, Mikhail A. Karymov, Yoshiko Kawano, Alexander Y. Lushnikov, Andrew Mikheikin, Vladimir N. Uversky, Yuri L Lyubchenko

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Protein misfolding is conformational transition dramatically facilitating the assembly of protein molecules into aggregates of various morphologies. Spontaneous formation of specific aggregates, mostly amyloid fibrils, was initially believed to be limited to proteins involved in the development of amyloidoses. However, recent studies show that, depending on conditions, the majority of proteins undergo structural transitions leading to the appearance of amyloidogenic intermediates followed by aggregate formation. Various techniques have been used to characterize the protein misfolding facilitating the aggregation process, but no direct evidence as to how such a conformational transition increases the intermolecular interactions has been obtained as of yet. We have applied atomic force microscopy (AFM) to follow the interaction between protein molecules as a function of pH. These studies were performed for three unrelated and structurally distinctive proteins, α-synuclein, amyloid β-peptide (Aβ) and lysozyme. It was shown that the attractive force between homologous protein molecules is minimal at physiological pH and increases dramatically at acidic pH. Moreover, the dependence of the pulling forces is sharp, suggesting a pH-dependent conformational transition within the protein. Parallel circular dichroism (CD) measurements performed for α-synuclein and Aβ revealed that the decrease in pH is accompanied by a sharp conformational transition from a random coil at neutral pH to the more ordered, predominantly β-sheet, structure at low pH. Importantly, the pH ranges for these conformational transitions coincide with those of pulling forces changes detected by AFM. In addition, protein self-assembly into filamentous aggregates studied by AFM imaging was shown to be facilitated at pH values corresponding to the maximum of pulling forces. Overall, these results indicate that proteins at acidic pH undergo structural transition into conformations responsible for the dramatic increase in interprotein interaction and promoting the formation of protein aggregates.

Original languageEnglish (US)
Pages (from-to)1028-1042
Number of pages15
JournalJournal of Molecular Biology
Volume354
Issue number5
DOIs
StatePublished - Dec 16 2005

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Atomic Force Microscopy
Spectrum Analysis
Proteins
Synucleins
Amyloidogenic Proteins
Amyloidosis
Muramidase
Circular Dichroism
Amyloid

Keywords

  • AFM
  • Amyloids
  • Force spectroscopy
  • Intermolecular interaction
  • Protein misfolding

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

McAllister, C., Karymov, M. A., Kawano, Y., Lushnikov, A. Y., Mikheikin, A., Uversky, V. N., & Lyubchenko, Y. L. (2005). Protein interactions and misfolding analyzed by AFM force spectroscopy. Journal of Molecular Biology, 354(5), 1028-1042. https://doi.org/10.1016/j.jmb.2005.10.012

Protein interactions and misfolding analyzed by AFM force spectroscopy. / McAllister, Chad; Karymov, Mikhail A.; Kawano, Yoshiko; Lushnikov, Alexander Y.; Mikheikin, Andrew; Uversky, Vladimir N.; Lyubchenko, Yuri L.

In: Journal of Molecular Biology, Vol. 354, No. 5, 16.12.2005, p. 1028-1042.

Research output: Contribution to journalArticle

McAllister, C, Karymov, MA, Kawano, Y, Lushnikov, AY, Mikheikin, A, Uversky, VN & Lyubchenko, YL 2005, 'Protein interactions and misfolding analyzed by AFM force spectroscopy', Journal of Molecular Biology, vol. 354, no. 5, pp. 1028-1042. https://doi.org/10.1016/j.jmb.2005.10.012
McAllister C, Karymov MA, Kawano Y, Lushnikov AY, Mikheikin A, Uversky VN et al. Protein interactions and misfolding analyzed by AFM force spectroscopy. Journal of Molecular Biology. 2005 Dec 16;354(5):1028-1042. https://doi.org/10.1016/j.jmb.2005.10.012
McAllister, Chad ; Karymov, Mikhail A. ; Kawano, Yoshiko ; Lushnikov, Alexander Y. ; Mikheikin, Andrew ; Uversky, Vladimir N. ; Lyubchenko, Yuri L. / Protein interactions and misfolding analyzed by AFM force spectroscopy. In: Journal of Molecular Biology. 2005 ; Vol. 354, No. 5. pp. 1028-1042.
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