Protein crystals can be incommensurately modulated

Jeffrey J. Lovelace, Cameron R. Murphy, Lee Daniels, Kartik Narayan, Clarence E. Schutt, Uno Lindberg, Christer Svensson, Gloria E Borgstahl

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin:actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin:actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected.

Original languageEnglish (US)
Pages (from-to)600-605
Number of pages6
JournalJournal of Applied Crystallography
Volume41
Issue number3
DOIs
StatePublished - Apr 19 2008

Fingerprint

Profilins
Actins
Diffraction patterns
proteins
diffraction patterns
Crystals
crystals
Proteins
Crystal lattices
integers
Sweden
X-Ray Diffraction
Modulation
modulation
X-Rays
crosslinking
crystal lattices
Crosslinking
ribbons
x rays

Keywords

  • Actin
  • Incommensurate crystallography
  • Modulated diffraction
  • Modulated protein crystals
  • Profilin
  • Satellite reflections

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lovelace, J. J., Murphy, C. R., Daniels, L., Narayan, K., Schutt, C. E., Lindberg, U., ... Borgstahl, G. E. (2008). Protein crystals can be incommensurately modulated. Journal of Applied Crystallography, 41(3), 600-605. https://doi.org/10.1107/S0021889808010716

Protein crystals can be incommensurately modulated. / Lovelace, Jeffrey J.; Murphy, Cameron R.; Daniels, Lee; Narayan, Kartik; Schutt, Clarence E.; Lindberg, Uno; Svensson, Christer; Borgstahl, Gloria E.

In: Journal of Applied Crystallography, Vol. 41, No. 3, 19.04.2008, p. 600-605.

Research output: Contribution to journalArticle

Lovelace, JJ, Murphy, CR, Daniels, L, Narayan, K, Schutt, CE, Lindberg, U, Svensson, C & Borgstahl, GE 2008, 'Protein crystals can be incommensurately modulated', Journal of Applied Crystallography, vol. 41, no. 3, pp. 600-605. https://doi.org/10.1107/S0021889808010716
Lovelace JJ, Murphy CR, Daniels L, Narayan K, Schutt CE, Lindberg U et al. Protein crystals can be incommensurately modulated. Journal of Applied Crystallography. 2008 Apr 19;41(3):600-605. https://doi.org/10.1107/S0021889808010716
Lovelace, Jeffrey J. ; Murphy, Cameron R. ; Daniels, Lee ; Narayan, Kartik ; Schutt, Clarence E. ; Lindberg, Uno ; Svensson, Christer ; Borgstahl, Gloria E. / Protein crystals can be incommensurately modulated. In: Journal of Applied Crystallography. 2008 ; Vol. 41, No. 3. pp. 600-605.
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