Protein co-isolated with human tissue factor impairs recovery of activity

Steven D Carson, S. E. Ross, R. A. Gramzinski

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Preparations of human tissue factor isolated by immunoaffinity chromatography contain variable amounts of 47,000 mol wt, 55,000 mol wt, and multimeric tissue factor when analyzed without reduction on polyacrylamide gels in sodium dodecyl sulfate (SDS). When analyzed after reduction, the 47,000 mol wt tissue factor apoprotein and a protein of about 12,000 mol wt are observed. Elution of tissue factor from polyacrylamide gel slices, followed by reassociation with lipids, restored proportionately much greater tissue factor activity with the 47,000-mol wt protein than with the 55,000-mol wt form. Cyanogen bromide cleavage at the single tissue factor methionine revealed that the 12,000-mol wt protein is associated with the carboxyl-terminal peptide derived from the 47,000-mol wt protein. These results reveal that association of the 12,000-mol wt protein with the cytoplasmic domain of tissue factor can modulate its activity in vitro.

Original languageEnglish (US)
Pages (from-to)520-523
Number of pages4
JournalBlood
Volume71
Issue number2
StatePublished - Jan 1 1988

Fingerprint

Thromboplastin
Recovery
Proteins
Cyanogen Bromide
Apoproteins
Chromatography
Sodium Dodecyl Sulfate
Methionine
Association reactions
Lipids
Peptides

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Carson, S. D., Ross, S. E., & Gramzinski, R. A. (1988). Protein co-isolated with human tissue factor impairs recovery of activity. Blood, 71(2), 520-523.

Protein co-isolated with human tissue factor impairs recovery of activity. / Carson, Steven D; Ross, S. E.; Gramzinski, R. A.

In: Blood, Vol. 71, No. 2, 01.01.1988, p. 520-523.

Research output: Contribution to journalArticle

Carson, SD, Ross, SE & Gramzinski, RA 1988, 'Protein co-isolated with human tissue factor impairs recovery of activity', Blood, vol. 71, no. 2, pp. 520-523.
Carson, Steven D ; Ross, S. E. ; Gramzinski, R. A. / Protein co-isolated with human tissue factor impairs recovery of activity. In: Blood. 1988 ; Vol. 71, No. 2. pp. 520-523.
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