Protein chromatography using a continuous stationary phase

Yiqi Yang, Ajoy Velayudhan, Christine M. Ladisch, Michael R. Ladisch

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A continuous stationary phase consisting of yarns woven into a fabric is rolled and packed into mechanically stable liquid chromatography columns. This work utilized yarns having a characteristic width of 200-400 μm, made from 10-20-μm fibers consisting of 95% poly(m-phenylene isophthalamide) and 5% poly(p-phenylene terephthalamide). Although loadings on this stationary phase were low at 4 mg/g for bovine serum albumin and 6 mg/g for β-galactosidase, this material shows the interesting characteristic of a leveling off of plate height at mobile phase velocities of 30-80 cm/min. This phenomenon is explained on the basis of a coupling argument whereby a fraction of the mobile phase flows through the intramatrix pore space, and convective transport through the pore space dominates transport by diffusion. A modified Van Deemter expression is derived and shown to fit plate height data for polyethylene glycol standards having molecular weights of 200 and 20 000. The characteristics of this continuous stationary phase at high eluent velocities are discussed conditions which give separation of immunoglobulin G, bovine serum albumin, insulin and β-galactosidase in 12 min are described.

Original languageEnglish (US)
Pages (from-to)169-180
Number of pages12
JournalJournal of Chromatography A
Volume598
Issue number2
DOIs
StatePublished - May 15 1992

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Galactosidases
Bovine Serum Albumin
Chromatography
Yarn
Phase velocity
Liquid chromatography
Liquid Chromatography
Proteins
Immunoglobulin G
Molecular Weight
Molecular weight
Insulin
Fibers
poly(4-phenylene terephthalamide)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Protein chromatography using a continuous stationary phase. / Yang, Yiqi; Velayudhan, Ajoy; Ladisch, Christine M.; Ladisch, Michael R.

In: Journal of Chromatography A, Vol. 598, No. 2, 15.05.1992, p. 169-180.

Research output: Contribution to journalArticle

Yang, Yiqi ; Velayudhan, Ajoy ; Ladisch, Christine M. ; Ladisch, Michael R. / Protein chromatography using a continuous stationary phase. In: Journal of Chromatography A. 1992 ; Vol. 598, No. 2. pp. 169-180.
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