Protective effect of preconditioning on ischemic heart and characterization of adenosine receptors in ischemic rabbit heart

Y. L. Li, R. R. He

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: To assess the role of adenosine receptors in the cardioprotective effect of preconditioning and the characterization of adenosine receptors in heart. Methods: In 18 anesthetized rabbits, myocardial ischemia was induced by occlusion of left descending coronary artery. ST segment elevation in epicardial electrogram was used as a criterion of acute ischemic injury. Myocardial adenosine receptors were measured using [3H]adenosine ligand. Results: After 60-min ischemia, epicardial ST segment elevation was higher in ischemia group (13.9 ± 0.6 mV) than in the ischemic preconditioning group (3.1 ± 0.5 mV). In membranes of the ventricular myocytes prepared from ischemic-preconditioning group, the density of adenosine receptors was higher than that of ischemia and sham ischemia groups (B(max) being 602 ± 40, 348 ± 28, and 335 ± 30 pmol/g protein, respectively). Affinity of the receptors for the [3H]adenosine was not different in the 3 groups (K(d) being 181 ± 18, 169 ± 21, and 196 ±24 nmol · L-1, respectively). Conclusion: Activation of adenosine receptors along with an increase in adenosine receptor density during ischemic-preconditioning provides the basis for adenosine to exert its protective effect on the ischemic heart.

Original languageEnglish (US)
Pages (from-to)505-508
Number of pages4
JournalActa Pharmacologica Sinica
Volume16
Issue number6
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Purinergic P1 Receptors
Rabbits
Adenosine
Ischemia
Muscle Cells
Myocardial Ischemia
Chemical activation
Coronary Vessels
Ligands
Membranes
Wounds and Injuries
Proteins

Keywords

  • cell membrane
  • electrocardiography
  • heart
  • myocardial ischemia
  • purinergic P receptors
  • radioligand assay

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology

Cite this

Protective effect of preconditioning on ischemic heart and characterization of adenosine receptors in ischemic rabbit heart. / Li, Y. L.; He, R. R.

In: Acta Pharmacologica Sinica, Vol. 16, No. 6, 01.01.1995, p. 505-508.

Research output: Contribution to journalArticle

@article{98d7882cb2cd4cf1920ba05e765731dd,
title = "Protective effect of preconditioning on ischemic heart and characterization of adenosine receptors in ischemic rabbit heart",
abstract = "Aim: To assess the role of adenosine receptors in the cardioprotective effect of preconditioning and the characterization of adenosine receptors in heart. Methods: In 18 anesthetized rabbits, myocardial ischemia was induced by occlusion of left descending coronary artery. ST segment elevation in epicardial electrogram was used as a criterion of acute ischemic injury. Myocardial adenosine receptors were measured using [3H]adenosine ligand. Results: After 60-min ischemia, epicardial ST segment elevation was higher in ischemia group (13.9 ± 0.6 mV) than in the ischemic preconditioning group (3.1 ± 0.5 mV). In membranes of the ventricular myocytes prepared from ischemic-preconditioning group, the density of adenosine receptors was higher than that of ischemia and sham ischemia groups (B(max) being 602 ± 40, 348 ± 28, and 335 ± 30 pmol/g protein, respectively). Affinity of the receptors for the [3H]adenosine was not different in the 3 groups (K(d) being 181 ± 18, 169 ± 21, and 196 ±24 nmol · L-1, respectively). Conclusion: Activation of adenosine receptors along with an increase in adenosine receptor density during ischemic-preconditioning provides the basis for adenosine to exert its protective effect on the ischemic heart.",
keywords = "cell membrane, electrocardiography, heart, myocardial ischemia, purinergic P receptors, radioligand assay",
author = "Li, {Y. L.} and He, {R. R.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "16",
pages = "505--508",
journal = "Zhongguo yao li xue bao = Acta pharmacologica Sinica",
issn = "1671-4083",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Protective effect of preconditioning on ischemic heart and characterization of adenosine receptors in ischemic rabbit heart

AU - Li, Y. L.

AU - He, R. R.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Aim: To assess the role of adenosine receptors in the cardioprotective effect of preconditioning and the characterization of adenosine receptors in heart. Methods: In 18 anesthetized rabbits, myocardial ischemia was induced by occlusion of left descending coronary artery. ST segment elevation in epicardial electrogram was used as a criterion of acute ischemic injury. Myocardial adenosine receptors were measured using [3H]adenosine ligand. Results: After 60-min ischemia, epicardial ST segment elevation was higher in ischemia group (13.9 ± 0.6 mV) than in the ischemic preconditioning group (3.1 ± 0.5 mV). In membranes of the ventricular myocytes prepared from ischemic-preconditioning group, the density of adenosine receptors was higher than that of ischemia and sham ischemia groups (B(max) being 602 ± 40, 348 ± 28, and 335 ± 30 pmol/g protein, respectively). Affinity of the receptors for the [3H]adenosine was not different in the 3 groups (K(d) being 181 ± 18, 169 ± 21, and 196 ±24 nmol · L-1, respectively). Conclusion: Activation of adenosine receptors along with an increase in adenosine receptor density during ischemic-preconditioning provides the basis for adenosine to exert its protective effect on the ischemic heart.

AB - Aim: To assess the role of adenosine receptors in the cardioprotective effect of preconditioning and the characterization of adenosine receptors in heart. Methods: In 18 anesthetized rabbits, myocardial ischemia was induced by occlusion of left descending coronary artery. ST segment elevation in epicardial electrogram was used as a criterion of acute ischemic injury. Myocardial adenosine receptors were measured using [3H]adenosine ligand. Results: After 60-min ischemia, epicardial ST segment elevation was higher in ischemia group (13.9 ± 0.6 mV) than in the ischemic preconditioning group (3.1 ± 0.5 mV). In membranes of the ventricular myocytes prepared from ischemic-preconditioning group, the density of adenosine receptors was higher than that of ischemia and sham ischemia groups (B(max) being 602 ± 40, 348 ± 28, and 335 ± 30 pmol/g protein, respectively). Affinity of the receptors for the [3H]adenosine was not different in the 3 groups (K(d) being 181 ± 18, 169 ± 21, and 196 ±24 nmol · L-1, respectively). Conclusion: Activation of adenosine receptors along with an increase in adenosine receptor density during ischemic-preconditioning provides the basis for adenosine to exert its protective effect on the ischemic heart.

KW - cell membrane

KW - electrocardiography

KW - heart

KW - myocardial ischemia

KW - purinergic P receptors

KW - radioligand assay

UR - http://www.scopus.com/inward/record.url?scp=0028788856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028788856&partnerID=8YFLogxK

M3 - Article

C2 - 8732043

AN - SCOPUS:0028788856

VL - 16

SP - 505

EP - 508

JO - Zhongguo yao li xue bao = Acta pharmacologica Sinica

JF - Zhongguo yao li xue bao = Acta pharmacologica Sinica

SN - 1671-4083

IS - 6

ER -