Protection of two PAI-2 mutants against TNF-α-mediated apoptosis

Yu Qing Zhang, Min Hou, Xia Wang, Xing Hui Sun, Ping Li, Yun Song Zhu

Research output: Contribution to journalArticle

Abstract

Purpose: To investigate different protective activities of two PAI-2 mutants against TNF-α mediated apopotosis and to explore the effect of the spatial structure of CD interhelical region of PAI-2 on its anti-apoptotic activity. Methods: Two PAI-2 mutants, PAI-2D and PAI-2M, were cloned into eukaryotic expression vector respectively and were transfected into Hela cells. After being analyzed by RT-PCR, the positive cell strains were obtained. The anti-apoptotic effects of these two mutants were compared by MTT assay and computer-aided homology modeling revealed some changes of their spatial structures. Results: PAI-2M could protect cells from the cytotoxic effects of TNF-α, while PAI-2D lost its protection function. Mostly PAI-2M retained the spatial structure of wild type PAI-2, while PAI-2D showed obvious difference, especially in the CD interhelical region. Conclusions: The protection of PAI-2 from TNF-α induced apoptosis depends on the spatial integrality of CD interhelical region of PAI-2, and the PENF domain (PENF 73-76in the CD interhelical region is not so important that it could be substituted.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume30
Issue number4
StatePublished - Jul 1 2003

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Plasminogen Activator Inhibitor 2
Apoptosis
HeLa Cells
Polymerase Chain Reaction

Keywords

  • Apoptisis
  • Mutant
  • Plasminogen activator inhibitor type-2
  • Tumor necrosis factur-α

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zhang, Y. Q., Hou, M., Wang, X., Sun, X. H., Li, P., & Zhu, Y. S. (2003). Protection of two PAI-2 mutants against TNF-α-mediated apoptosis. Fudan University Journal of Medical Sciences, 30(4), 313-316.

Protection of two PAI-2 mutants against TNF-α-mediated apoptosis. / Zhang, Yu Qing; Hou, Min; Wang, Xia; Sun, Xing Hui; Li, Ping; Zhu, Yun Song.

In: Fudan University Journal of Medical Sciences, Vol. 30, No. 4, 01.07.2003, p. 313-316.

Research output: Contribution to journalArticle

Zhang, YQ, Hou, M, Wang, X, Sun, XH, Li, P & Zhu, YS 2003, 'Protection of two PAI-2 mutants against TNF-α-mediated apoptosis', Fudan University Journal of Medical Sciences, vol. 30, no. 4, pp. 313-316.
Zhang, Yu Qing ; Hou, Min ; Wang, Xia ; Sun, Xing Hui ; Li, Ping ; Zhu, Yun Song. / Protection of two PAI-2 mutants against TNF-α-mediated apoptosis. In: Fudan University Journal of Medical Sciences. 2003 ; Vol. 30, No. 4. pp. 313-316.
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