Prostaglandin hydroperoxidase-catalyzed activation of certain N-substituted aryl renal and bladder carcinogens

T. V. Zenser, S. M. Cohen, M. B. Mattammal

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Certain carcinogens are thought to induce renal and bladder cancer following metabolic activation. We propose a model system for this activation and provide supporting experimental evidence. This model proposes that renal and bladder carcinogens' entry into the urinary tract is facilitated, that carcinogens are activated by the prostaglandin hydroperoxidase activity of prostaglandin endoperoxide synthetase (PES), and that activation results in covalent binding to nucleic acids which can initiate carcinogenesis. Benzidine and the 5-nitrofuran HMN were shown to inhibit uptake of organic anions and cations, respectively. Carcinogen binding to DNA was dependent upon specific unsaturated fatty acid substrates and prevented by specific inhibitors of PES, i.e., aspirin. Activation with organic peroxides or H2O2 was inhibited by antioxidants but not aspirin. Horseradish peroxidase (HRP) metabolized benzidine but not ANFT. Acetaminophen and the 5-nitrofurans ANFT and HMN prevented PES 14C-benzidine metabolism. However, only acetaminophen inhibited HRP metabolism of benzidine. The only aerobic metabolism we have observed of 5-nitrofurans is PES-catalyzed. Aspirin (0.5% in the diet) inhibited rat bladder hyperplastic lesions induced by feeding 0.1% or 0.2% FANFT for 6 or 12 weeks. Aspirin reduced bladder prostaglandin synthesis and PES metabolism of FANFT. After one year of an ongoing long-term study, gross examination reveals bladder tumors in 85% of the rats fed 0.2% FANFT and in only 37% of the rats fed FANFT plus 0.5% aspirin.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalEnvironmental Health Perspectives
VolumeVol. 49
StatePublished - Jan 1 1983

Fingerprint

FANFT
carcinogen
Prostaglandin-Endoperoxide Synthases
Carcinogens
Aspirin
Nitrofurans
Urinary Bladder
Metabolism
metabolism
Chemical activation
Kidney
Rats
Horseradish Peroxidase
Acetaminophen
Urinary Bladder Neoplasms
nucleic acid
lesion
tumor
antioxidant
Kidney Neoplasms

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Prostaglandin hydroperoxidase-catalyzed activation of certain N-substituted aryl renal and bladder carcinogens. / Zenser, T. V.; Cohen, S. M.; Mattammal, M. B.

In: Environmental Health Perspectives, Vol. Vol. 49, 01.01.1983, p. 33-41.

Research output: Contribution to journalArticle

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abstract = "Certain carcinogens are thought to induce renal and bladder cancer following metabolic activation. We propose a model system for this activation and provide supporting experimental evidence. This model proposes that renal and bladder carcinogens' entry into the urinary tract is facilitated, that carcinogens are activated by the prostaglandin hydroperoxidase activity of prostaglandin endoperoxide synthetase (PES), and that activation results in covalent binding to nucleic acids which can initiate carcinogenesis. Benzidine and the 5-nitrofuran HMN were shown to inhibit uptake of organic anions and cations, respectively. Carcinogen binding to DNA was dependent upon specific unsaturated fatty acid substrates and prevented by specific inhibitors of PES, i.e., aspirin. Activation with organic peroxides or H2O2 was inhibited by antioxidants but not aspirin. Horseradish peroxidase (HRP) metabolized benzidine but not ANFT. Acetaminophen and the 5-nitrofurans ANFT and HMN prevented PES 14C-benzidine metabolism. However, only acetaminophen inhibited HRP metabolism of benzidine. The only aerobic metabolism we have observed of 5-nitrofurans is PES-catalyzed. Aspirin (0.5{\%} in the diet) inhibited rat bladder hyperplastic lesions induced by feeding 0.1{\%} or 0.2{\%} FANFT for 6 or 12 weeks. Aspirin reduced bladder prostaglandin synthesis and PES metabolism of FANFT. After one year of an ongoing long-term study, gross examination reveals bladder tumors in 85{\%} of the rats fed 0.2{\%} FANFT and in only 37{\%} of the rats fed FANFT plus 0.5{\%} aspirin.",
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