Prostaglandin F increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum

The possible regulation of blood flow at an early stage of luteolysis

Koumei Shirasuna, Sho Watanabe, Takayuki Asahi, Missaka P B Wijayagunawardane, Kiemi Sasahara, Chao Jiang, Motozumi Matsui, Motoki Sasaki, Takashi Shimizu, John S Davis, Akio Miyamoto

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Prostaglandin F (PGF) released from the uterus causes alterations in luteal blood flow, reduces progesterone secretion, and induces luteolysis in the bovine corpus luteum (CL). We have recently discovered that luteal blood flow in the periphery of the mature CL acutely increases coincidently with pulsatile increases in a metabolite of PGF (PGFM). In this study, we characterized changes in regional luteal blood flow together with regional alterations in endothelial nitric oxide synthase (eNOS) expression during spontaneous luteolysis and in response to PGF. Smooth muscle actin-positive blood vessels larger than 20 μm were observed mainly in the periphery of mature CL. PGF receptor was localized to luteal cells and large blood vessels in the periphery of mid-CL. PGF acutely stimulated eNOS expression in the periphery but not in the center of mature CL. Injection of the NO donor S-nitroso-N-acetylpenicillamine into CL induced an acute increase in luteal blood flow and shortened the estrous cycle. In contrast, injection of the NOS inhibitor L-NAME into CL completely suppressed the acute increase in luteal blood flow induced by PGF and delayed the onset of luteolysis. In conclusion, PGF has a site-restricted action depending on not only luteal phase but also the region in the CL. PGF stimulates eNOS expression, vasodilation of blood vessels, and increased luteal blood flow in periphery of mature CL. Furthermore, the increased blood flow is mediated by NO, suggesting that the acute increase in peripheral blood flow to CL is one of the first physiological indicators of NO action in response to PGF.

Original languageEnglish (US)
Pages (from-to)527-539
Number of pages13
JournalReproduction
Volume135
Issue number4
DOIs
StatePublished - Apr 1 2008

Fingerprint

Luteolysis
Dinoprost
Nitric Oxide Synthase Type III
Corpus Luteum
Blood Vessels
S-Nitroso-N-Acetylpenicillamine
Luteal Cells
Injections
Estrous Cycle
Luteal Phase
NG-Nitroarginine Methyl Ester
Regional Blood Flow

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Cite this

Prostaglandin F increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum : The possible regulation of blood flow at an early stage of luteolysis. / Shirasuna, Koumei; Watanabe, Sho; Asahi, Takayuki; Wijayagunawardane, Missaka P B; Sasahara, Kiemi; Jiang, Chao; Matsui, Motozumi; Sasaki, Motoki; Shimizu, Takashi; Davis, John S; Miyamoto, Akio.

In: Reproduction, Vol. 135, No. 4, 01.04.2008, p. 527-539.

Research output: Contribution to journalArticle

Shirasuna, K, Watanabe, S, Asahi, T, Wijayagunawardane, MPB, Sasahara, K, Jiang, C, Matsui, M, Sasaki, M, Shimizu, T, Davis, JS & Miyamoto, A 2008, 'Prostaglandin F increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: The possible regulation of blood flow at an early stage of luteolysis', Reproduction, vol. 135, no. 4, pp. 527-539. https://doi.org/10.1530/REP-07-0496
Shirasuna, Koumei ; Watanabe, Sho ; Asahi, Takayuki ; Wijayagunawardane, Missaka P B ; Sasahara, Kiemi ; Jiang, Chao ; Matsui, Motozumi ; Sasaki, Motoki ; Shimizu, Takashi ; Davis, John S ; Miyamoto, Akio. / Prostaglandin F increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum : The possible regulation of blood flow at an early stage of luteolysis. In: Reproduction. 2008 ; Vol. 135, No. 4. pp. 527-539.
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abstract = "Prostaglandin F2α (PGF2α) released from the uterus causes alterations in luteal blood flow, reduces progesterone secretion, and induces luteolysis in the bovine corpus luteum (CL). We have recently discovered that luteal blood flow in the periphery of the mature CL acutely increases coincidently with pulsatile increases in a metabolite of PGF2α (PGFM). In this study, we characterized changes in regional luteal blood flow together with regional alterations in endothelial nitric oxide synthase (eNOS) expression during spontaneous luteolysis and in response to PGF2α. Smooth muscle actin-positive blood vessels larger than 20 μm were observed mainly in the periphery of mature CL. PGF2α receptor was localized to luteal cells and large blood vessels in the periphery of mid-CL. PGF2α acutely stimulated eNOS expression in the periphery but not in the center of mature CL. Injection of the NO donor S-nitroso-N-acetylpenicillamine into CL induced an acute increase in luteal blood flow and shortened the estrous cycle. In contrast, injection of the NOS inhibitor L-NAME into CL completely suppressed the acute increase in luteal blood flow induced by PGF2α and delayed the onset of luteolysis. In conclusion, PGF2α has a site-restricted action depending on not only luteal phase but also the region in the CL. PGF2α stimulates eNOS expression, vasodilation of blood vessels, and increased luteal blood flow in periphery of mature CL. Furthermore, the increased blood flow is mediated by NO, suggesting that the acute increase in peripheral blood flow to CL is one of the first physiological indicators of NO action in response to PGF2α.",
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AU - Jiang, Chao

AU - Matsui, Motozumi

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AU - Shimizu, Takashi

AU - Davis, John S

AU - Miyamoto, Akio

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