Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration

An Zeng, Hua Li, Longhua Guo, Xin Gao, Sean McKinney, Yongfu Wang, Zulin Yu, Jungeun Park, Craig Semerad, Eric Ross, Li Chun Cheng, Erin Davies, Kai Lei, Wei Wang, Anoja Perera, Kate Hall, Allison Peak, Andrew Box, Alejandro Sánchez Alvarado

Research output: Contribution to journalArticle

  • 1 Citations

Abstract

Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single-cell transplantation to address this long-standing issue. Large-scale scRNA-seq of sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein and marked by a conserved cell-surface protein-coding gene, tetraspanin 1 (tspan-1). tspan-1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody, rescued the viability of lethally irradiated animals after single-cell transplantation. The first prospective isolation of an adult PSC bridges a conceptual dichotomy between functionally and molecularly defined neoblasts, shedding light on mechanisms governing in vivo pluripotency and a source of regeneration in animals. Video Abstract: [Figure presented] Transplantation of a single pluripotent neoblast can restore regenerative ability and viability to lethally irradiated Planaria.

LanguageEnglish (US)
Pages1593-1608.e20
JournalCell
Volume173
Issue number7
DOIs
StatePublished - Jun 14 2018

Fingerprint

Thermionic emission
Pluripotent Stem Cells
Adult Stem Cells
Delta wing aircraft
Regeneration
RNA Sequence Analysis
Photosensitizers
Cell Transplantation
Planarians
Human Body
Membrane Proteins
Homeostasis
Transplantation
Messenger RNA
Antibodies
Genes
Proteins

Keywords

  • planaria
  • pluripotent
  • prospective isolation
  • regeneration
  • single cell transplantation
  • stem cells
  • tetraspanin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zeng, A., Li, H., Guo, L., Gao, X., McKinney, S., Wang, Y., ... Sánchez Alvarado, A. (2018). Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. Cell, 173(7), 1593-1608.e20. DOI: 10.1016/j.cell.2018.05.006

Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. / Zeng, An; Li, Hua; Guo, Longhua; Gao, Xin; McKinney, Sean; Wang, Yongfu; Yu, Zulin; Park, Jungeun; Semerad, Craig; Ross, Eric; Cheng, Li Chun; Davies, Erin; Lei, Kai; Wang, Wei; Perera, Anoja; Hall, Kate; Peak, Allison; Box, Andrew; Sánchez Alvarado, Alejandro.

In: Cell, Vol. 173, No. 7, 14.06.2018, p. 1593-1608.e20.

Research output: Contribution to journalArticle

Zeng, A, Li, H, Guo, L, Gao, X, McKinney, S, Wang, Y, Yu, Z, Park, J, Semerad, C, Ross, E, Cheng, LC, Davies, E, Lei, K, Wang, W, Perera, A, Hall, K, Peak, A, Box, A & Sánchez Alvarado, A 2018, 'Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration' Cell, vol. 173, no. 7, pp. 1593-1608.e20. DOI: 10.1016/j.cell.2018.05.006
Zeng A, Li H, Guo L, Gao X, McKinney S, Wang Y et al. Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. Cell. 2018 Jun 14;173(7):1593-1608.e20. Available from, DOI: 10.1016/j.cell.2018.05.006
Zeng, An ; Li, Hua ; Guo, Longhua ; Gao, Xin ; McKinney, Sean ; Wang, Yongfu ; Yu, Zulin ; Park, Jungeun ; Semerad, Craig ; Ross, Eric ; Cheng, Li Chun ; Davies, Erin ; Lei, Kai ; Wang, Wei ; Perera, Anoja ; Hall, Kate ; Peak, Allison ; Box, Andrew ; Sánchez Alvarado, Alejandro. / Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. In: Cell. 2018 ; Vol. 173, No. 7. pp. 1593-1608.e20
@article{406c2ac0fa16405dba19c7880a64b691,
title = "Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration",
abstract = "Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single-cell transplantation to address this long-standing issue. Large-scale scRNA-seq of sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein and marked by a conserved cell-surface protein-coding gene, tetraspanin 1 (tspan-1). tspan-1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody, rescued the viability of lethally irradiated animals after single-cell transplantation. The first prospective isolation of an adult PSC bridges a conceptual dichotomy between functionally and molecularly defined neoblasts, shedding light on mechanisms governing in vivo pluripotency and a source of regeneration in animals. Video Abstract: [Figure presented] Transplantation of a single pluripotent neoblast can restore regenerative ability and viability to lethally irradiated Planaria.",
keywords = "planaria, pluripotent, prospective isolation, regeneration, single cell transplantation, stem cells, tetraspanin",
author = "An Zeng and Hua Li and Longhua Guo and Xin Gao and Sean McKinney and Yongfu Wang and Zulin Yu and Jungeun Park and Craig Semerad and Eric Ross and Cheng, {Li Chun} and Erin Davies and Kai Lei and Wei Wang and Anoja Perera and Kate Hall and Allison Peak and Andrew Box and {S{\'a}nchez Alvarado}, Alejandro",
year = "2018",
month = "6",
day = "14",
doi = "10.1016/j.cell.2018.05.006",
language = "English (US)",
volume = "173",
pages = "1593--1608.e20",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",

}

TY - JOUR

T1 - Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration

AU - Zeng,An

AU - Li,Hua

AU - Guo,Longhua

AU - Gao,Xin

AU - McKinney,Sean

AU - Wang,Yongfu

AU - Yu,Zulin

AU - Park,Jungeun

AU - Semerad,Craig

AU - Ross,Eric

AU - Cheng,Li Chun

AU - Davies,Erin

AU - Lei,Kai

AU - Wang,Wei

AU - Perera,Anoja

AU - Hall,Kate

AU - Peak,Allison

AU - Box,Andrew

AU - Sánchez Alvarado,Alejandro

PY - 2018/6/14

Y1 - 2018/6/14

N2 - Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single-cell transplantation to address this long-standing issue. Large-scale scRNA-seq of sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein and marked by a conserved cell-surface protein-coding gene, tetraspanin 1 (tspan-1). tspan-1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody, rescued the viability of lethally irradiated animals after single-cell transplantation. The first prospective isolation of an adult PSC bridges a conceptual dichotomy between functionally and molecularly defined neoblasts, shedding light on mechanisms governing in vivo pluripotency and a source of regeneration in animals. Video Abstract: [Figure presented] Transplantation of a single pluripotent neoblast can restore regenerative ability and viability to lethally irradiated Planaria.

AB - Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single-cell transplantation to address this long-standing issue. Large-scale scRNA-seq of sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein and marked by a conserved cell-surface protein-coding gene, tetraspanin 1 (tspan-1). tspan-1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody, rescued the viability of lethally irradiated animals after single-cell transplantation. The first prospective isolation of an adult PSC bridges a conceptual dichotomy between functionally and molecularly defined neoblasts, shedding light on mechanisms governing in vivo pluripotency and a source of regeneration in animals. Video Abstract: [Figure presented] Transplantation of a single pluripotent neoblast can restore regenerative ability and viability to lethally irradiated Planaria.

KW - planaria

KW - pluripotent

KW - prospective isolation

KW - regeneration

KW - single cell transplantation

KW - stem cells

KW - tetraspanin

UR - http://www.scopus.com/inward/record.url?scp=85048097498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048097498&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2018.05.006

DO - 10.1016/j.cell.2018.05.006

M3 - Article

VL - 173

SP - 1593-1608.e20

JO - Cell

T2 - Cell

JF - Cell

SN - 0092-8674

IS - 7

ER -