Prosomatostatin-I is processed to somatostatin-26 and somatostatin-14 in the pancreas of the bowfin, Amia calva

Yunxia Wang, John H. Youson, J. Michael Conlon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

With the exception of the Agnatha (lampreys and hagfishes), somatostatin-14 is the predominant molecular form of somatostatin in the pancreas of species from all classes of vertebrates yet studied. The pancreas of the holostean fish, Amia calva (bowfin; order Amiiformes) contained somatostatin-like immunoreactivity that was resolved by reversed phase HPLC in two components. The primary structure of the more abundant peptide (somatostatin-26) was established as: Ser-Ala-Asn-Pro-Ala5-Leu-Ala-Pro-Arg-Glu10-Arg-Lys-Ala-Gly-Cys15-Lys-Asn-Phe-Phe-Trp20-Lys-Thr-Phe-Thr-Ser25-Cys. This amino acid sequence shows one substitution (Leu for Met at position 6) and two deletions compared with mammalian somatostatin-28. The minor component was identical to somatostatin-14. The data show that the pathway of post-translational processing of prosomatostatin-I in the bowfin pancreas is appreciably different from the corresponding pathway in teleost fish and higher vertebrates.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalRegulatory Peptides
Volume47
Issue number1
DOIs
StatePublished - Aug 13 1993

Fingerprint

Somatostatin
Pancreas
Fish
Vertebrates
Fishes
Somatostatin-28
Hagfishes
Lampreys
Amino Acid Sequence
Substitution reactions
High Pressure Liquid Chromatography
Amino Acids
Peptides
Processing
prosomatostatin
somatostatin 26

Keywords

  • Amiiformes
  • Pancreas
  • Post-translational processing
  • Prosomatostatin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Prosomatostatin-I is processed to somatostatin-26 and somatostatin-14 in the pancreas of the bowfin, Amia calva. / Wang, Yunxia; Youson, John H.; Conlon, J. Michael.

In: Regulatory Peptides, Vol. 47, No. 1, 13.08.1993, p. 33-39.

Research output: Contribution to journalArticle

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