Properties of the functioning of the promoter of the microcin c51 operon under different conditions of escherichia coli cell growth

A. M. Veselovskii, Dmitri Fomenko, A. Z. Metlitskaya, V. A. Lipasova, I. A. Khmel

Research output: Contribution to journalArticle

Abstract

The level of transcription from the promoter of the microcin C51 operon (P^c) depends on the growth phase of Escherichia coli cells: transcription proceeds with low efficiency at the exponential phase of growth and with higher efficiency when growth of cells is delayed during entry into the stationary phase. The functioning of P^ was studied in cells grown in different media by a single-copy construct, which contained the cloned promoter region of the microcin C51 operon and the promoterless lac operon. A decrease in the rate of cell growth caused by changes in the sole carbon source in minimal medium correlated with an increase in the level of transcription from the PmcC promoter at the exponential phase of growth; the expression of Pm:c-lac during cell entry into the stationary phase was higher under unfavorable medium conditions. The use of composite rich media impaired this feature. The addition of Meucine (100 |ig/ml) to the medium decreased the expression of P-foc in wildtype cells carrying the birp mutation. A further increase in leucine concentration and the presence of other amino acids in the medium enhanced transcription that started from P,^ during cell entry into the stationary growth phase. The capacity of the P^c promoter and of the wild-type lacZ gene promoter was virtually the same upon IPTG induction. A mutation in the ompR gene did not markedly influence transcription started from ?

Original languageEnglish (US)
Pages (from-to)1055-1062
Number of pages8
JournalGenetika
Volume37
Issue number8
StatePublished - Dec 1 2001

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Operon
Escherichia coli
Growth
Lac Operon
Isopropyl Thiogalactoside
Mutation
microcin
Genetic Promoter Regions
Leucine
Carbon
Amino Acids
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Veselovskii, A. M., Fomenko, D., Metlitskaya, A. Z., Lipasova, V. A., & Khmel, I. A. (2001). Properties of the functioning of the promoter of the microcin c51 operon under different conditions of escherichia coli cell growth. Genetika, 37(8), 1055-1062.

Properties of the functioning of the promoter of the microcin c51 operon under different conditions of escherichia coli cell growth. / Veselovskii, A. M.; Fomenko, Dmitri; Metlitskaya, A. Z.; Lipasova, V. A.; Khmel, I. A.

In: Genetika, Vol. 37, No. 8, 01.12.2001, p. 1055-1062.

Research output: Contribution to journalArticle

Veselovskii, AM, Fomenko, D, Metlitskaya, AZ, Lipasova, VA & Khmel, IA 2001, 'Properties of the functioning of the promoter of the microcin c51 operon under different conditions of escherichia coli cell growth', Genetika, vol. 37, no. 8, pp. 1055-1062.
Veselovskii, A. M. ; Fomenko, Dmitri ; Metlitskaya, A. Z. ; Lipasova, V. A. ; Khmel, I. A. / Properties of the functioning of the promoter of the microcin c51 operon under different conditions of escherichia coli cell growth. In: Genetika. 2001 ; Vol. 37, No. 8. pp. 1055-1062.
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