Properties of the chlorella receptor for the virus PBCV-11

Russel H. Meints, Dwight E. Burbank, James L. Van Etten, Derek T.A. Lamport

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The virus PBCV-1 attached rapidly, specifically, and irreversibly to the external surface of cell walls of its host, a unicellular, eukaryotic Chlorella-like green alga. Attachment was pH and salt dependent. Each cell contained at least 5 × 10SU PBCV-1 binding sites and Scatchard analysis indicated that each cell could adsorb 5000 PBCV-1 particles. The PBCV-1 receptor was unaffected by extraction with organic solvents, detergents, high salts, or treatment with several proteases as well as the polysaccharide degrading enzymes, cellulase and pectinase. In contrast, acid and alkali treatments of walls at high temperatures and treatment with an enzyme preparation from PBCV-1 lysates destroyed the virus receptor. We suspect that the receptor is a carbohydrate.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalVirology
Volume164
Issue number1
DOIs
StatePublished - May 1988

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Virus Receptors
Chlorella
Salts
Polygalacturonase
Chlorophyta
Cellulase
Alkalies
Enzymes
Detergents
Cell Wall
Polysaccharides
Peptide Hydrolases
Binding Sites
Carbohydrates
Viruses
Temperature
Acids

ASJC Scopus subject areas

  • Virology

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Properties of the chlorella receptor for the virus PBCV-11. / Meints, Russel H.; Burbank, Dwight E.; Van Etten, James L.; Lamport, Derek T.A.

In: Virology, Vol. 164, No. 1, 05.1988, p. 15-21.

Research output: Contribution to journalArticle

Meints, Russel H. ; Burbank, Dwight E. ; Van Etten, James L. ; Lamport, Derek T.A. / Properties of the chlorella receptor for the virus PBCV-11. In: Virology. 1988 ; Vol. 164, No. 1. pp. 15-21.
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