Properties of marmoset T lymphocytes cultured in vitro

Donald R Johnson, L. Falk, R. Yang, F. Deinhardt

Research output: Contribution to journalArticle

Abstract

Marmoset splenic or circulating lymphocytes can be transformed in vitro with Herpesvirus ateles (HVA); the transformed lymphocytes grow as continuous lymphoblastoid cell cultures and can be cultivated indefinitely. These transformed cells possessed properties of T lymphocytes: 30-70% of cells formed E rosettes, all lacked surface Ig but reacted with a specific antimarmoset T lymphocyte serum. Cells of 10 lymphoblastoid cultures, derived after transformation in vitro of marmoset circulating lymphocytes, were analyzed for 5 surface characteristics: anti T lymphocyte serum reacted in a 51Cr assay with cells of all 10 cultures at 42-86% cytotoxicity (this serum reacts only slightly with cultured B lymphocytes). Cells of 5 lines formed E rosettes (11-65% reactive cells) and EAC rosettes (18-55% reactive cells) and cells of the other 5 cultures formed neither E or EAC rosettes. None of the cells possessed detectable surface Ig, however 5-10% of cells were positive for Fc receptors. These results show that cultured lymphoblastoid cells express T and/or B markers, however E or EAC rosette formation is not consistent criteria for classification of lymphocytes and therefore other markers must be determined. In contrast to lymphocytes transformed with Epstein Barr virus that are always B lymphocytes and express B lymphocyte characteristics, HVA appears to transform T lymphocytes which express variable characteristics.

Original languageEnglish (US)
JournalFederation Proceedings
Volume34
Issue number3
StatePublished - Jan 1 1975

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Callithrix
T-Lymphocytes
Lymphocytes
Rhadinovirus
B-Lymphocytes
Serum
In Vitro Techniques
Rosette Formation
Fc Receptors
Human Herpesvirus 4
Cultured Cells
Cell Culture Techniques
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Johnson, D. R., Falk, L., Yang, R., & Deinhardt, F. (1975). Properties of marmoset T lymphocytes cultured in vitro. Federation Proceedings, 34(3).

Properties of marmoset T lymphocytes cultured in vitro. / Johnson, Donald R; Falk, L.; Yang, R.; Deinhardt, F.

In: Federation Proceedings, Vol. 34, No. 3, 01.01.1975.

Research output: Contribution to journalArticle

Johnson, DR, Falk, L, Yang, R & Deinhardt, F 1975, 'Properties of marmoset T lymphocytes cultured in vitro', Federation Proceedings, vol. 34, no. 3.
Johnson DR, Falk L, Yang R, Deinhardt F. Properties of marmoset T lymphocytes cultured in vitro. Federation Proceedings. 1975 Jan 1;34(3).
Johnson, Donald R ; Falk, L. ; Yang, R. ; Deinhardt, F. / Properties of marmoset T lymphocytes cultured in vitro. In: Federation Proceedings. 1975 ; Vol. 34, No. 3.
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