Promoting apoptosis: A novel activity associated with the cyclin- dependent kinase inhibitor p27

Yu Katayose, Min Kim, Amol N.S. Rakkar, Zhuangwu Li, Kenneth H. Cowan, Prem Seth

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

p27(Kip1), a cyclin-dependent kinase inhibitor, is recognized as a negative regulator of the cell cycle. In this paper, we report that overexpression of p27(Kip1) triggers apoptosis in several different human cancer cell lines. Using a recombinant adenoviral vector that expresses p27(Kip1) (Adp27), we found that overexpression of p27(Kip1) in MDA-MB-231 breast cancer cells induces apoptosis that was seen by a number of different techniques, including flow cytometry and in situ terminal deoxynucleotidyl transferase-mediated nick end labeling, flow cytometric assay for sub-G1 population, and 4',6-diamindino-2-phenylindole staining. Cleavage of poly(ADP-ribose) polymerase and degradation of cyclin B1, events that are known to be associated with apoptosis, were also observed following overexpression of p27(Kip1). This is the first report indicating a role for p27(Kip1) in induction of apoptosis.

Original languageEnglish (US)
Pages (from-to)5441-5445
Number of pages5
JournalCancer Research
Volume57
Issue number24
StatePublished - Dec 15 1997

Fingerprint

Cyclin-Dependent Kinase Inhibitor p27
Apoptosis
Cyclin B1
DNA Nucleotidylexotransferase
Poly(ADP-ribose) Polymerases
Cyclin-Dependent Kinases
Cell Cycle
Flow Cytometry
Staining and Labeling
Breast Neoplasms
Cell Line
Population
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Katayose, Y., Kim, M., Rakkar, A. N. S., Li, Z., Cowan, K. H., & Seth, P. (1997). Promoting apoptosis: A novel activity associated with the cyclin- dependent kinase inhibitor p27. Cancer Research, 57(24), 5441-5445.

Promoting apoptosis : A novel activity associated with the cyclin- dependent kinase inhibitor p27. / Katayose, Yu; Kim, Min; Rakkar, Amol N.S.; Li, Zhuangwu; Cowan, Kenneth H.; Seth, Prem.

In: Cancer Research, Vol. 57, No. 24, 15.12.1997, p. 5441-5445.

Research output: Contribution to journalArticle

Katayose, Y, Kim, M, Rakkar, ANS, Li, Z, Cowan, KH & Seth, P 1997, 'Promoting apoptosis: A novel activity associated with the cyclin- dependent kinase inhibitor p27', Cancer Research, vol. 57, no. 24, pp. 5441-5445.
Katayose, Yu ; Kim, Min ; Rakkar, Amol N.S. ; Li, Zhuangwu ; Cowan, Kenneth H. ; Seth, Prem. / Promoting apoptosis : A novel activity associated with the cyclin- dependent kinase inhibitor p27. In: Cancer Research. 1997 ; Vol. 57, No. 24. pp. 5441-5445.
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