Promises and pitfalls of stem cell therapy for promotion of bone healing

John G Sharp, Barbara O Kane Murphy, John D. Jackson, Susan K. Brusnahan, Anne Kessinger, James R. Neff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

There is promise in combining stem cells with allogeneic bone matrix to promote bone healing. Murine bone marrow, peripheral blood, and compact bone cells were transplanted ectopically under the kidney capsule in mice, alone or in combination with allogeneic matrix products: powder and putty to determine their bone forming potential in comparison to transplanted femoral bone fragments and long-term cultured bone marrow cells. The end point was the amount of bone formed as determined by quantitative histology. Mononuclear cells from marrow, peripheral blood, or bone alone transplanted under the kidney capsule did not form bone. Mononuclear cell populations did not combine readily with matrix products and there was in vivo migration of the transplanted combinations. Kidney subcapsular transplanted cultured bone marrow cells formed bone in proportion to the culture period, but after 9 weeks, the extent was only 20% by area of that of similarly transplanted femoral bone fragments. An inductive stimulus for bone formation seemed necessary. Osteoprogenitor cells were not detected in significant numbers in blood unless high doses of cytokines were administered. A better definition of the optimal cell populations and manipulations required for promotion of bone healing is needed along with new (transplant) models that allow for cell tracking. Much work remains to overcome current pitfalls in the use of stem cells to promote allograft integration and bone healing. Level of Evidence: Therapeutic study, Level V (expert opinion).

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalClinical Orthopaedics and Related Research
Issue number435
DOIs
StatePublished - Jun 2005

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Cell- and Tissue-Based Therapy
Stem Cells
Bone and Bones
Thigh
Kidney
Bone Marrow Cells
Capsules
Cultured Cells
Bone Marrow
Cell Tracking
Bone Matrix
Expert Testimony
Osteogenesis
Powders
Population
Allografts
Histology
Cytokines
Transplants

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Promises and pitfalls of stem cell therapy for promotion of bone healing. / Sharp, John G; Murphy, Barbara O Kane; Jackson, John D.; Brusnahan, Susan K.; Kessinger, Anne; Neff, James R.

In: Clinical Orthopaedics and Related Research, No. 435, 06.2005, p. 52-61.

Research output: Contribution to journalArticle

Sharp, John G ; Murphy, Barbara O Kane ; Jackson, John D. ; Brusnahan, Susan K. ; Kessinger, Anne ; Neff, James R. / Promises and pitfalls of stem cell therapy for promotion of bone healing. In: Clinical Orthopaedics and Related Research. 2005 ; No. 435. pp. 52-61.
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