Prolongation of allogeneic skin graft survival by injection of anti-Ly49A monoclonal antibody YE1/48

Tian Yu Yang, Yimin Sun, Alan Norman Langnas, Yong Zhao

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Ly49A receptors expressed on NK, NKT, and T cells play inhibitory roles in regulating the immune responses in vivo and in vitro. Whether or not injection of anti-Ly49A monoclonal antibody (mAb) YE1/48 can block allograft rejection has not been evaluated. Balb/c mouse recipients received intraperitoneal injections of YE1/48 mAb (0.5 mg) or control mAb or phosphate-buffered saline on days -1 and 10. On day 0, fully MHC-mismatched allogeneic C57BL/6 (B6) skin grafts were implanted. The skin graft survival and anti-donor humoral responses were observed. Whereas allogeneic B6 skin grafts survived 14 days in isotopy control antibody-treated or nontreated Balb/c mice, injection of YE1/48 mAb significantly prolonged B6 skin graft survival to 19 days (P < 0.0005). Injection of YE1/48 mAb into presensitized Balb/c recipients did not significantly delay B6 skin graft rejection. On the other hand, after depleting recipient NK, NKT, and some cytotoxic T cells by injection of anti-asialo GM1, YE1/48 failed to prolong B6 skin graft survival in Balb/c recipients. The present studies indicate that injection of YE1/48 mAb significantly delays allogeneic skin graft rejection in nonsensitized recipients but not in sensitized recipients. The presence of NK, NKT cells, and some cytotoxic T cells may be essential for YE1/48-mediated immunosuppression in vivo.

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalClinical Immunology
Volume106
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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Keywords

  • Graft
  • Immune response
  • Transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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