Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase

Richard G MacDonald, Edmund A. Gianferrari, Wendell W. Leavitt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

These studies were done to determine if the progesterone-induced estrogen receptor-regulatory factor (ReRF) in hamster uterus is 17β-hydroxysteroid dehydrogenase (17β-HSD), i.e. that rapid loss of nuclear estrogen receptor (Re) might be due to enhanced estradiol oxidation to estrone catalyzed by 17β-HSD. Treatment of proestrous hamsters with progesterone (~25 mg/kg BW) for either 2 h or 4 h had no effect on 17β-HSD activity measured as the rate of conversion of [6,7-3H]estradiol to [3H]estrone by whole uterine homogenstes at 35°C. During this same time interval, progesterone treatment increased the rate of inactivation of the occupied form of nuclear Re as determined during a 30 m1n incubation of uterine nuclear extract in vitro at 36°C. Since we previously demonstrated that such in vitro Re-inactivating activity represents ReRF, the present studies show that ReRF is not 17β-HSD or a modifier of that enzyme.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalSteroids
Volume40
Issue number4
DOIs
StatePublished - Jan 1 1982

Fingerprint

Estrogen Receptors
Progesterone
Estrone
Cricetinae
Estradiol
Cytoplasmic and Nuclear Receptors
Uterus
Oxidation
3(17)-hydroxysteroid dehydrogenase
Enzymes
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase. / MacDonald, Richard G; Gianferrari, Edmund A.; Leavitt, Wendell W.

In: Steroids, Vol. 40, No. 4, 01.01.1982, p. 465-473.

Research output: Contribution to journalArticle

MacDonald, Richard G ; Gianferrari, Edmund A. ; Leavitt, Wendell W. / Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase. In: Steroids. 1982 ; Vol. 40, No. 4. pp. 465-473.
@article{c931731d558f430bac7ce1e1987f0ed5,
title = "Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase",
abstract = "These studies were done to determine if the progesterone-induced estrogen receptor-regulatory factor (ReRF) in hamster uterus is 17β-hydroxysteroid dehydrogenase (17β-HSD), i.e. that rapid loss of nuclear estrogen receptor (Re) might be due to enhanced estradiol oxidation to estrone catalyzed by 17β-HSD. Treatment of proestrous hamsters with progesterone (~25 mg/kg BW) for either 2 h or 4 h had no effect on 17β-HSD activity measured as the rate of conversion of [6,7-3H]estradiol to [3H]estrone by whole uterine homogenstes at 35°C. During this same time interval, progesterone treatment increased the rate of inactivation of the occupied form of nuclear Re as determined during a 30 m1n incubation of uterine nuclear extract in vitro at 36°C. Since we previously demonstrated that such in vitro Re-inactivating activity represents ReRF, the present studies show that ReRF is not 17β-HSD or a modifier of that enzyme.",
author = "MacDonald, {Richard G} and Gianferrari, {Edmund A.} and Leavitt, {Wendell W.}",
year = "1982",
month = "1",
day = "1",
doi = "10.1016/0039-128X(82)90023-X",
language = "English (US)",
volume = "40",
pages = "465--473",
journal = "Steroids",
issn = "0039-128X",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase

AU - MacDonald, Richard G

AU - Gianferrari, Edmund A.

AU - Leavitt, Wendell W.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - These studies were done to determine if the progesterone-induced estrogen receptor-regulatory factor (ReRF) in hamster uterus is 17β-hydroxysteroid dehydrogenase (17β-HSD), i.e. that rapid loss of nuclear estrogen receptor (Re) might be due to enhanced estradiol oxidation to estrone catalyzed by 17β-HSD. Treatment of proestrous hamsters with progesterone (~25 mg/kg BW) for either 2 h or 4 h had no effect on 17β-HSD activity measured as the rate of conversion of [6,7-3H]estradiol to [3H]estrone by whole uterine homogenstes at 35°C. During this same time interval, progesterone treatment increased the rate of inactivation of the occupied form of nuclear Re as determined during a 30 m1n incubation of uterine nuclear extract in vitro at 36°C. Since we previously demonstrated that such in vitro Re-inactivating activity represents ReRF, the present studies show that ReRF is not 17β-HSD or a modifier of that enzyme.

AB - These studies were done to determine if the progesterone-induced estrogen receptor-regulatory factor (ReRF) in hamster uterus is 17β-hydroxysteroid dehydrogenase (17β-HSD), i.e. that rapid loss of nuclear estrogen receptor (Re) might be due to enhanced estradiol oxidation to estrone catalyzed by 17β-HSD. Treatment of proestrous hamsters with progesterone (~25 mg/kg BW) for either 2 h or 4 h had no effect on 17β-HSD activity measured as the rate of conversion of [6,7-3H]estradiol to [3H]estrone by whole uterine homogenstes at 35°C. During this same time interval, progesterone treatment increased the rate of inactivation of the occupied form of nuclear Re as determined during a 30 m1n incubation of uterine nuclear extract in vitro at 36°C. Since we previously demonstrated that such in vitro Re-inactivating activity represents ReRF, the present studies show that ReRF is not 17β-HSD or a modifier of that enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0020314554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020314554&partnerID=8YFLogxK

U2 - 10.1016/0039-128X(82)90023-X

DO - 10.1016/0039-128X(82)90023-X

M3 - Article

VL - 40

SP - 465

EP - 473

JO - Steroids

JF - Steroids

SN - 0039-128X

IS - 4

ER -