Progesterone-induced estrogen receptor-regulatory factor is not 17β-hydroxysteroid dehydrogenase

Richard G. Macdonald, Edmund A. Gianferrari, Wendell W. Leavitt

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

These studies were done to determine if the progesterone-induced estrogen receptor-regulatory factor (ReRF) in hamster uterus is 17β-hydroxysteroid dehydrogenase (17β-HSD), i.e. that rapid loss of nuclear estrogen receptor (Re) might be due to enhanced estradiol oxidation to estrone catalyzed by 17β-HSD. Treatment of proestrous hamsters with progesterone (~25 mg/kg BW) for either 2 h or 4 h had no effect on 17β-HSD activity measured as the rate of conversion of [6,7-3H]estradiol to [3H]estrone by whole uterine homogenstes at 35°C. During this same time interval, progesterone treatment increased the rate of inactivation of the occupied form of nuclear Re as determined during a 30 m1n incubation of uterine nuclear extract in vitro at 36°C. Since we previously demonstrated that such in vitro Re-inactivating activity represents ReRF, the present studies show that ReRF is not 17β-HSD or a modifier of that enzyme.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalSteroids
Volume40
Issue number4
DOIs
StatePublished - Oct 1982

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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