Productive infection and bICP0 early promoter activity of bovine herpesvirus 1 are stimulated by E2F1

Aspen Workman, Clinton Jones

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bovine herpesvirus 1 (BoHV-1) is an important viral pathogen of cattle. Like other members of the subfamily Alphaherpesvirinae, BoHV-1 establishes latency in sensory neurons and has the potential to reactivate from latency. Dexamethasone (DEX) treatment of latently infected calves or rabbits consistently leads to reactivation from latency. The BoHV-1 transcript encoding the infected cell protein 0 (bICP0) is consistently detected during reactivation from latency, in part because the bICP0 early promoter is activated by DEX. During DEX-induced reactivation from latency, cyclin expression is stimulated in infected sensory neurons. Cyclindependent kinase activity phosphorylates Rb (retinoblastoma tumor suppressor gene product) family proteins and consequently releases the E2F family of transcription factors, suggesting that E2F family members stimulate productive infection and/or reactivation from latency. In this study, we provide evidence that repression of E2F1 by a specific small interfering RNA (siRNA) reduced productive infection approximately 5-fold. E2F1 or E2F2 stimulated bICP0 early promoter activity at least 100-fold in transient transfection assays. Two E2F-responsive regions (ERR) were identified within the early promoter, with one adjacent to the TATA box (ERR1) and one approximately 600 bp upstream from the TATA box (ERR2). Mobility shift assays suggested that E2F interacts with ERR1 and ERR2. E2F1 protein levels were increased at late times after infection, which correlated with enhanced binding to a consensus E2F binding site, ERR1, or ERR2. Collectively, these studies suggest that E2F1 stimulates productive infection and bICP0 early promoter activity, in part because E2F family members interact with ERR1 and ERR2.

Original languageEnglish (US)
Pages (from-to)6308-6317
Number of pages10
JournalJournal of virology
Volume84
Issue number13
DOIs
StatePublished - Jul 1 2010

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
promoter regions
dexamethasone
Infection
E2F Transcription Factors
infection
Dexamethasone
TATA box
TATA Box
Proteins
proteins
sensory neurons
Sensory Receptor Cells
cells
Alphaherpesvirinae
Cyclins
tumor suppressor genes
cyclin-dependent kinase
Retinoblastoma

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Productive infection and bICP0 early promoter activity of bovine herpesvirus 1 are stimulated by E2F1. / Workman, Aspen; Jones, Clinton.

In: Journal of virology, Vol. 84, No. 13, 01.07.2010, p. 6308-6317.

Research output: Contribution to journalArticle

Workman, Aspen ; Jones, Clinton. / Productive infection and bICP0 early promoter activity of bovine herpesvirus 1 are stimulated by E2F1. In: Journal of virology. 2010 ; Vol. 84, No. 13. pp. 6308-6317.
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