Production of scatter factor-like activity by a nitrosamine-induced pancreatic cancer cell line

Masahiko Hirota, Hiroshi Egami, Stefano Corra, Hideki Fujii, William G Chaney, A Angie Rizzino, Parviz M. Pour

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Two hamster pancreatic cancer cell lines, PC-1 and PC1.0, established from N-nitrosobis(2-oxopropyl)amine-induced pancreatic ductal/ductular adenocarcinomas exhibit different growth patterns. PC-1 cells, which produce well differentiated adenocarcinomas in vitro after allogeneic inoculation, form cell aggregates and characteristic island-like structures in vitro. PC1.0 cells, which produce poorly differentiated tumors in vivo, form dispersed colonies in vitro. Conditioned medium prepared from PC1.0 cells inhibits PC-1 cells from forming island-like colonies. The conditioned medium also prevents several human pancreatic carcinoma cell lines, HPAF, CD11 and CD18, from forming compact colonies. These properties are similar to those described previously as scatter factors. The scatter factor-like activity is heat-labile, acid-stable, non-dialyzable, trypsin sensitive and unaffected by reducing agents. The activity is not suppressed by addition of heparin, and it does not bind to heparin. In addition, the scatter phenomenon is not reproduced by acidic or basic fibroblast growth factor, epidermal growth factor or transforming growth factor-β1. Based on these findings, it appears that the scattering activity produced by PC1.0 cells differs from the scatter factors that have been identified in other systems.

Original languageEnglish (US)
Pages (from-to)259-264
Number of pages6
JournalCarcinogenesis
Volume14
Issue number2
DOIs
StatePublished - Feb 1 1993

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Nitrosamines
Hepatocyte Growth Factor
Scatter
Pancreatic Neoplasms
Cancer
Cells
Cell Line
Line
Cell
Reducing agents
Fibroblasts
Tumors
Amines
Growth Factors
nitrosobis(2-oxopropyl)amine
Scattering
Conditioned Culture Medium
Heparin
Islands
Acids

ASJC Scopus subject areas

  • Cancer Research

Cite this

Production of scatter factor-like activity by a nitrosamine-induced pancreatic cancer cell line. / Hirota, Masahiko; Egami, Hiroshi; Corra, Stefano; Fujii, Hideki; Chaney, William G; Rizzino, A Angie; Pour, Parviz M.

In: Carcinogenesis, Vol. 14, No. 2, 01.02.1993, p. 259-264.

Research output: Contribution to journalArticle

Hirota, Masahiko ; Egami, Hiroshi ; Corra, Stefano ; Fujii, Hideki ; Chaney, William G ; Rizzino, A Angie ; Pour, Parviz M. / Production of scatter factor-like activity by a nitrosamine-induced pancreatic cancer cell line. In: Carcinogenesis. 1993 ; Vol. 14, No. 2. pp. 259-264.
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