Production of reversible papillary proliferation of the urinary bladder in rats

T. Shirai, Samuel Monroe Cohen, S. Fukushima, N. Ito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A rapid and reproducible method for the production of ulcers and regenerative hyperplasia of the bladder of rats is described. Ulceration was produced by applying a steel rod, 5 mm in diameter, frozen in dry ice acetone (-78°), to the serosal surface of the bladder. Preliminary experiments had determined that an exposure of 2 sec, twice, with 5 sec between the exposures while the rod was reimmersed in dry ice acetone resulted in ulceration. Sequential histologic observations showed that the ulcers were accompanied by necrosis of the entire thickness of the bladder wall and that after 1 day, there was a marked exudate consisting of polymorphonuclear leukocytes, red blood cells, fibrin, and cellular debris. Regenerative hyperplasia was evident near the edge of the ulcer by the second day. The hyperplasia increased until day 5 when it became marked including a fibrovascular core indicating papillary and nodular formation. By the 10th day no ulcer remained. Hyperplastic mucosal proliferation was slight to moderate by day 7, and only minimum hyperplasia was observed at 15 days although slight fibrosis in the muscular and submucosal layers was observed.

Original languageEnglish (US)
Pages (from-to)521-522
Number of pages2
JournalGann, The Japanese Journal of Cancer Research
Volume68
Issue number4
StatePublished - Dec 1 1977

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Ulcer
Hyperplasia
Urinary Bladder
Dry Ice
Acetone
Steel
Exudates and Transudates
Fibrin
Neutrophils
Fibrosis
Necrosis
Erythrocytes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Production of reversible papillary proliferation of the urinary bladder in rats. / Shirai, T.; Cohen, Samuel Monroe; Fukushima, S.; Ito, N.

In: Gann, The Japanese Journal of Cancer Research, Vol. 68, No. 4, 01.12.1977, p. 521-522.

Research output: Contribution to journalArticle

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N2 - A rapid and reproducible method for the production of ulcers and regenerative hyperplasia of the bladder of rats is described. Ulceration was produced by applying a steel rod, 5 mm in diameter, frozen in dry ice acetone (-78°), to the serosal surface of the bladder. Preliminary experiments had determined that an exposure of 2 sec, twice, with 5 sec between the exposures while the rod was reimmersed in dry ice acetone resulted in ulceration. Sequential histologic observations showed that the ulcers were accompanied by necrosis of the entire thickness of the bladder wall and that after 1 day, there was a marked exudate consisting of polymorphonuclear leukocytes, red blood cells, fibrin, and cellular debris. Regenerative hyperplasia was evident near the edge of the ulcer by the second day. The hyperplasia increased until day 5 when it became marked including a fibrovascular core indicating papillary and nodular formation. By the 10th day no ulcer remained. Hyperplastic mucosal proliferation was slight to moderate by day 7, and only minimum hyperplasia was observed at 15 days although slight fibrosis in the muscular and submucosal layers was observed.

AB - A rapid and reproducible method for the production of ulcers and regenerative hyperplasia of the bladder of rats is described. Ulceration was produced by applying a steel rod, 5 mm in diameter, frozen in dry ice acetone (-78°), to the serosal surface of the bladder. Preliminary experiments had determined that an exposure of 2 sec, twice, with 5 sec between the exposures while the rod was reimmersed in dry ice acetone resulted in ulceration. Sequential histologic observations showed that the ulcers were accompanied by necrosis of the entire thickness of the bladder wall and that after 1 day, there was a marked exudate consisting of polymorphonuclear leukocytes, red blood cells, fibrin, and cellular debris. Regenerative hyperplasia was evident near the edge of the ulcer by the second day. The hyperplasia increased until day 5 when it became marked including a fibrovascular core indicating papillary and nodular formation. By the 10th day no ulcer remained. Hyperplastic mucosal proliferation was slight to moderate by day 7, and only minimum hyperplasia was observed at 15 days although slight fibrosis in the muscular and submucosal layers was observed.

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