Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds

Meghna R. Malik, Wenyu Yang, Nii Patterson, Jihong Tang, Rachel L. Wellinghoff, Mary L. Preuss, Claire Burkitt, Nirmala Sharma, Yuanyuan Ji, Joseph M. Jez, Oliver P. Peoples, Jan G. Jaworski, Edgar B. Cahoon, Kristi D. Snell

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Poly-3-hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed-specific promoters for expression of transgenes. Genes encoding PHB biosynthetic enzymes were modified at the N-terminus to encode a plastid targeting signal. PHB levels of up to 15% of the mature seed weight were measured in single sacrificed T1 seeds with a genetic construct containing the oleosin and glycinin promoters. A more detailed analysis of the PHB production potential of two of the best performing binary vectors in a Camelina line bred for larger seed size yielded lines containing up to 15% polymer in mature T2 seeds. Transmission electron microscopy showed the presence of distinct granules of PHB in the seeds. PHB production had varying effects on germination, emergence and survival of seedlings. Once true leaves formed, plants grew normally and were able to set seeds. PHB synthesis lowered the total oil but not the protein content of engineered seeds. A change in the oil fatty acid profile was also observed. High molecular weight polymer was produced with weight-averaged molecular weights varying between 600 000 and 1 500 000, depending on the line. Select lines were advanced to later generations yielding a line with 13.7% PHB in T4 seeds. The levels of polymer produced in this study are the highest reported to date in a seed and are an important step forward for commercializing an oilseed-based platform for PHB production.

Original languageEnglish (US)
Pages (from-to)675-688
Number of pages14
JournalPlant Biotechnology Journal
Volume13
Issue number5
DOIs
StatePublished - Jun 1 2015

Fingerprint

Camelina sativa
Plastids
plastids
Seeds
seeds
polymers
Polymers
Oils
promoter regions
poly-3-hydroxybutyrate
poly-beta-hydroxybutyrate
Camelina
oleosin
Molecular Weight
molecular weight
glycinin
oils
Weights and Measures
oilseeds
Plant Leaves

Keywords

  • Bioplastics
  • Camelina sativa
  • Oilseeds
  • Poly-3-hydroxybutyrate
  • Polyhydroxyalkanoate
  • Polyhydroxybutyrate

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Malik, M. R., Yang, W., Patterson, N., Tang, J., Wellinghoff, R. L., Preuss, M. L., ... Snell, K. D. (2015). Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds. Plant Biotechnology Journal, 13(5), 675-688. https://doi.org/10.1111/pbi.12290

Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds. / Malik, Meghna R.; Yang, Wenyu; Patterson, Nii; Tang, Jihong; Wellinghoff, Rachel L.; Preuss, Mary L.; Burkitt, Claire; Sharma, Nirmala; Ji, Yuanyuan; Jez, Joseph M.; Peoples, Oliver P.; Jaworski, Jan G.; Cahoon, Edgar B.; Snell, Kristi D.

In: Plant Biotechnology Journal, Vol. 13, No. 5, 01.06.2015, p. 675-688.

Research output: Contribution to journalArticle

Malik, MR, Yang, W, Patterson, N, Tang, J, Wellinghoff, RL, Preuss, ML, Burkitt, C, Sharma, N, Ji, Y, Jez, JM, Peoples, OP, Jaworski, JG, Cahoon, EB & Snell, KD 2015, 'Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds', Plant Biotechnology Journal, vol. 13, no. 5, pp. 675-688. https://doi.org/10.1111/pbi.12290
Malik MR, Yang W, Patterson N, Tang J, Wellinghoff RL, Preuss ML et al. Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds. Plant Biotechnology Journal. 2015 Jun 1;13(5):675-688. https://doi.org/10.1111/pbi.12290
Malik, Meghna R. ; Yang, Wenyu ; Patterson, Nii ; Tang, Jihong ; Wellinghoff, Rachel L. ; Preuss, Mary L. ; Burkitt, Claire ; Sharma, Nirmala ; Ji, Yuanyuan ; Jez, Joseph M. ; Peoples, Oliver P. ; Jaworski, Jan G. ; Cahoon, Edgar B. ; Snell, Kristi D. / Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds. In: Plant Biotechnology Journal. 2015 ; Vol. 13, No. 5. pp. 675-688.
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