Production of fatty acid components of meadowfoam oil in somatic soybean embryos

E. B. Cahoon, E. F. Marillia, K. L. Stecca, S. E. Hall, D. C. Taylor, A. J. Kinney

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The seed oil of meadowfoam (Limnanthes alba) and other Limnanthes spp. is enriched in the unusual fatty acid Δ5-eicosenoic acid (20:1Δ5). This fatty acid has physical and chemical properties that make the seed oil of these plants useful for a number of industrial applications. An expressed sequence tag approach was used to identify cDNAs for enzymes involved in the biosynthesis of 20:1Δ5). By random sequencing of a library prepared from developing Limnanthes douglasii seeds, a class of cDNAs was identified that encode a homolog of acyl-coenzyme A (CoA) desaturases found in animals, fungi, and cyanobacteria. Expression of a cDNA for the L. douglasii acyl-CoA desaturase homolog in somatic soybean (Glycine max) embryos behind a strong seed-specific promoter resulted in the accumulation of Δ5-hexadecenoic acid to amounts of 2% to 3% (w/w) of the total fatty acids of single embryos. Δ5-Octadecenoic acid and 20:1Δ5 also composed <1% (w/w) each of the total fatty acids of these embryos. In addition, cDNAs were identified from the L. douglasii expressed sequence tags that encode a homolog of fatty acid elongase 1 (FAE1), a β-ketoacyl-CoA synthase that catalyzes the initial step of very long-chain fatty acid synthesis. Expression of the L. douglassi FAE1 homolog in somatic soybean embryos was accompanied by the accumulation of C20 and C22 fatty acids, principally as eicosanoic acid, to amounts of 18% (w/w) of the total fatty acids of single embryos. To partially reconstruct the biosynthetic pathway of 20:1Δ5 in transgenic plant tissues, cDNAs for the L. douglasii acyl-CoA desaturase and FAE1 were co-expressed in somatic soybean embryos. In the resulting transgenic embryos, 20:1Δ5 and Δ5-docosenoic acid composed up to 12% of the total fatty acids.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalPlant physiology
Volume124
Issue number1
StatePublished - Oct 3 2000

Fingerprint

meadowfoam oil
Soybeans
embryo (plant)
Fatty Acids
Embryonic Structures
soybeans
Limnanthes douglasii
fatty acids
Complementary DNA
Acyl Coenzyme A
acyl coenzyme A
Seeds
Limnanthes
Expressed Sequence Tags
acids
Erucic Acids
Eicosanoic Acids
expressed sequence tags
docosenoic acids
Limnanthes alba

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Cahoon, E. B., Marillia, E. F., Stecca, K. L., Hall, S. E., Taylor, D. C., & Kinney, A. J. (2000). Production of fatty acid components of meadowfoam oil in somatic soybean embryos. Plant physiology, 124(1), 243-251.

Production of fatty acid components of meadowfoam oil in somatic soybean embryos. / Cahoon, E. B.; Marillia, E. F.; Stecca, K. L.; Hall, S. E.; Taylor, D. C.; Kinney, A. J.

In: Plant physiology, Vol. 124, No. 1, 03.10.2000, p. 243-251.

Research output: Contribution to journalArticle

Cahoon, EB, Marillia, EF, Stecca, KL, Hall, SE, Taylor, DC & Kinney, AJ 2000, 'Production of fatty acid components of meadowfoam oil in somatic soybean embryos', Plant physiology, vol. 124, no. 1, pp. 243-251.
Cahoon EB, Marillia EF, Stecca KL, Hall SE, Taylor DC, Kinney AJ. Production of fatty acid components of meadowfoam oil in somatic soybean embryos. Plant physiology. 2000 Oct 3;124(1):243-251.
Cahoon, E. B. ; Marillia, E. F. ; Stecca, K. L. ; Hall, S. E. ; Taylor, D. C. ; Kinney, A. J. / Production of fatty acid components of meadowfoam oil in somatic soybean embryos. In: Plant physiology. 2000 ; Vol. 124, No. 1. pp. 243-251.
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abstract = "The seed oil of meadowfoam (Limnanthes alba) and other Limnanthes spp. is enriched in the unusual fatty acid Δ5-eicosenoic acid (20:1Δ5). This fatty acid has physical and chemical properties that make the seed oil of these plants useful for a number of industrial applications. An expressed sequence tag approach was used to identify cDNAs for enzymes involved in the biosynthesis of 20:1Δ5). By random sequencing of a library prepared from developing Limnanthes douglasii seeds, a class of cDNAs was identified that encode a homolog of acyl-coenzyme A (CoA) desaturases found in animals, fungi, and cyanobacteria. Expression of a cDNA for the L. douglasii acyl-CoA desaturase homolog in somatic soybean (Glycine max) embryos behind a strong seed-specific promoter resulted in the accumulation of Δ5-hexadecenoic acid to amounts of 2{\%} to 3{\%} (w/w) of the total fatty acids of single embryos. Δ5-Octadecenoic acid and 20:1Δ5 also composed <1{\%} (w/w) each of the total fatty acids of these embryos. In addition, cDNAs were identified from the L. douglasii expressed sequence tags that encode a homolog of fatty acid elongase 1 (FAE1), a β-ketoacyl-CoA synthase that catalyzes the initial step of very long-chain fatty acid synthesis. Expression of the L. douglassi FAE1 homolog in somatic soybean embryos was accompanied by the accumulation of C20 and C22 fatty acids, principally as eicosanoic acid, to amounts of 18{\%} (w/w) of the total fatty acids of single embryos. To partially reconstruct the biosynthetic pathway of 20:1Δ5 in transgenic plant tissues, cDNAs for the L. douglasii acyl-CoA desaturase and FAE1 were co-expressed in somatic soybean embryos. In the resulting transgenic embryos, 20:1Δ5 and Δ5-docosenoic acid composed up to 12{\%} of the total fatty acids.",
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