Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

A. Adachi, H. E. Gendelman, S. Koenig, T. Folks, R. Willey, A. Rabson, M. A. Martin

Research output: Contribution to journalArticle

2160 Citations (Scopus)

Abstract

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

Original languageEnglish (US)
Pages (from-to)284-291
Number of pages8
JournalJournal of virology
Volume59
Issue number2
StatePublished - Nov 19 1986

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Retroviridae
acquired immunodeficiency syndrome
Acquired Immunodeficiency Syndrome
Clone Cells
transfection
clones
virion
Virion
Transfection
CD4-positive T-lymphocytes
cell lines
CD4-Positive T-Lymphocytes
Mink
Cell Line
mink
Viral RNA
Viral Proteins
cells
carcinoma
colon

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. / Adachi, A.; Gendelman, H. E.; Koenig, S.; Folks, T.; Willey, R.; Rabson, A.; Martin, M. A.

In: Journal of virology, Vol. 59, No. 2, 19.11.1986, p. 284-291.

Research output: Contribution to journalArticle

Adachi, A. ; Gendelman, H. E. ; Koenig, S. ; Folks, T. ; Willey, R. ; Rabson, A. ; Martin, M. A. / Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. In: Journal of virology. 1986 ; Vol. 59, No. 2. pp. 284-291.
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AU - Willey, R.

AU - Rabson, A.

AU - Martin, M. A.

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