Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

A. Adachi, H. E. Gendelman, S. Koenig, T. Folks, R. Willey, A. Rabson, M. A. Martin

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Abstract

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

Original languageEnglish (US)
Pages (from-to)284-291
Number of pages8
JournalJournal of virology
Volume59
Issue number2
StatePublished - Nov 19 1986

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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