Producing primate embryonic stem cells by somatic cell nuclear transfer

J. A. Byrne, D. A. Pedersen, L. L. Clepper, M. Nelson, W. G. Sanger, S. Gokhale, D. P. Wolf, S. M. Mitalipov

Research output: Contribution to journalArticle

436 Citations (Scopus)

Abstract

Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

Original languageEnglish (US)
Pages (from-to)497-502
Number of pages6
JournalNature
Volume450
Issue number7169
DOIs
StatePublished - Nov 21 2007

Fingerprint

Embryonic Stem Cells
Primates
Cell Line
Pluripotent Stem Cells
DNA
Blastocyst
Macaca mulatta
Mitochondrial DNA
Oocytes
Embryonic Development
Organism Cloning
Immune System
Stem Cells
Embryonic Structures
Fibroblasts
Tissue Donors
Skin
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Byrne, J. A., Pedersen, D. A., Clepper, L. L., Nelson, M., Sanger, W. G., Gokhale, S., ... Mitalipov, S. M. (2007). Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature, 450(7169), 497-502. https://doi.org/10.1038/nature06357

Producing primate embryonic stem cells by somatic cell nuclear transfer. / Byrne, J. A.; Pedersen, D. A.; Clepper, L. L.; Nelson, M.; Sanger, W. G.; Gokhale, S.; Wolf, D. P.; Mitalipov, S. M.

In: Nature, Vol. 450, No. 7169, 21.11.2007, p. 497-502.

Research output: Contribution to journalArticle

Byrne, JA, Pedersen, DA, Clepper, LL, Nelson, M, Sanger, WG, Gokhale, S, Wolf, DP & Mitalipov, SM 2007, 'Producing primate embryonic stem cells by somatic cell nuclear transfer', Nature, vol. 450, no. 7169, pp. 497-502. https://doi.org/10.1038/nature06357
Byrne JA, Pedersen DA, Clepper LL, Nelson M, Sanger WG, Gokhale S et al. Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature. 2007 Nov 21;450(7169):497-502. https://doi.org/10.1038/nature06357
Byrne, J. A. ; Pedersen, D. A. ; Clepper, L. L. ; Nelson, M. ; Sanger, W. G. ; Gokhale, S. ; Wolf, D. P. ; Mitalipov, S. M. / Producing primate embryonic stem cells by somatic cell nuclear transfer. In: Nature. 2007 ; Vol. 450, No. 7169. pp. 497-502.
@article{04ba0e5885c44f4692b3da1f8f196883,
title = "Producing primate embryonic stem cells by somatic cell nuclear transfer",
abstract = "Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.",
author = "Byrne, {J. A.} and Pedersen, {D. A.} and Clepper, {L. L.} and M. Nelson and Sanger, {W. G.} and S. Gokhale and Wolf, {D. P.} and Mitalipov, {S. M.}",
year = "2007",
month = "11",
day = "21",
doi = "10.1038/nature06357",
language = "English (US)",
volume = "450",
pages = "497--502",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7169",

}

TY - JOUR

T1 - Producing primate embryonic stem cells by somatic cell nuclear transfer

AU - Byrne, J. A.

AU - Pedersen, D. A.

AU - Clepper, L. L.

AU - Nelson, M.

AU - Sanger, W. G.

AU - Gokhale, S.

AU - Wolf, D. P.

AU - Mitalipov, S. M.

PY - 2007/11/21

Y1 - 2007/11/21

N2 - Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

AB - Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

UR - http://www.scopus.com/inward/record.url?scp=36549033276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36549033276&partnerID=8YFLogxK

U2 - 10.1038/nature06357

DO - 10.1038/nature06357

M3 - Article

C2 - 18004281

AN - SCOPUS:36549033276

VL - 450

SP - 497

EP - 502

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7169

ER -