Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth

P. Tolstoshev, R. A. Berg, Stephen Israel Rennard, K. H. Bradley, B. C. Trapnell, R. G. Crystal

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The production of procollagen molecules by human diploid fetal lung fibroblasts (HFL-1 cells) remains constant in both rapid and stationary growth phases. However, log phase cells degrade 3-fold more newly synthesized collagen inside the cell prior to secretion than do stationary phase cells. Procollagen mRNA levels, measured by hybridization with a type I procollagen mRNA-specific complementary DNA, are approximately 2-fold higher in confluent cells than in log phase cells. There are no significant differences in the ability of either log phase or confluent HFL-1 cell procollagen mRNA to be translated in an in vitro cell-free translation system. Therefore, the ability of HFL-1 cell to maintain constant collagen production irrespective of the growth status the cells results from the combined action of a number of regulatory mechanisms, including changes in procollagen mRNA levels, the utilization of procollagen mRNA, and intracellular procollagen degradation.

Original languageEnglish (US)
Pages (from-to)3135-3140
Number of pages6
JournalJournal of Biological Chemistry
Volume256
Issue number6
StatePublished - Dec 1 1981

Fingerprint

Procollagen
Fibroblasts
Human Activities
Lung
Messenger RNA
Growth
Collagen
Collagen Type I
Cell-Free System
Complementary DNA
Diploidy
Degradation
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tolstoshev, P., Berg, R. A., Rennard, S. I., Bradley, K. H., Trapnell, B. C., & Crystal, R. G. (1981). Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. Journal of Biological Chemistry, 256(6), 3135-3140.

Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. / Tolstoshev, P.; Berg, R. A.; Rennard, Stephen Israel; Bradley, K. H.; Trapnell, B. C.; Crystal, R. G.

In: Journal of Biological Chemistry, Vol. 256, No. 6, 01.12.1981, p. 3135-3140.

Research output: Contribution to journalArticle

Tolstoshev, P, Berg, RA, Rennard, SI, Bradley, KH, Trapnell, BC & Crystal, RG 1981, 'Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth', Journal of Biological Chemistry, vol. 256, no. 6, pp. 3135-3140.
Tolstoshev, P. ; Berg, R. A. ; Rennard, Stephen Israel ; Bradley, K. H. ; Trapnell, B. C. ; Crystal, R. G. / Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. In: Journal of Biological Chemistry. 1981 ; Vol. 256, No. 6. pp. 3135-3140.
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