Probing subunit interactions in glutathione S-transferases using affinity labeling

Roberta F. Colman, Joseph J. Barycki, Melissa A. Vargo, Jibo Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Affinity labeling, using reactive analogs of natural ligands, provides a powerful approach to probe for the existence of cooperative interactions between subunits as well as to locate specific xenobiotic binding sites in glutathione 5-transferases (GST). Thus, 3β-(iodoacetoxy)-dehydroisoandrosterone (3β-IDA) and 17-(iodoacetoxy)estradiol-3-sulfate (17β-IES) function as affinity labels of the nonsubstrate steroid binding site of rat liver GST, isozyme 1-1. Modification of Cys17 or Cys111 on Subunit A prevents reaction of the same cysteine on Subunit B. Interactions between the subunits of both the 4-4 and 1-1 isozymes are also illustrated by photoaffinity labeling of the active sites of these homodimers by glutathionyl S[4-(succinimidyl)-benzophenone], an analog of the product of glutathione and xenobiotic substrate.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalChemico-Biological Interactions
Volume133
Issue number1-3
StatePublished - Feb 28 2001

Fingerprint

Xenobiotics
Glutathione Transferase
Labeling
Isoenzymes
Glutathione
Binding Sites
Affinity Labels
Transferases
Liver
Cysteine
Rats
Catalytic Domain
Steroids
Ligands
Substrates
GS-Succ-BP
estradiol-3-sulfate
3-(iodoacetoxy)dehydroisoandrosterone

Keywords

  • Affinity labeling
  • Glutathione S-transferase
  • Subunit interaction

ASJC Scopus subject areas

  • Toxicology

Cite this

Colman, R. F., Barycki, J. J., Vargo, M. A., & Wang, J. (2001). Probing subunit interactions in glutathione S-transferases using affinity labeling. Chemico-Biological Interactions, 133(1-3), 159-162.

Probing subunit interactions in glutathione S-transferases using affinity labeling. / Colman, Roberta F.; Barycki, Joseph J.; Vargo, Melissa A.; Wang, Jibo.

In: Chemico-Biological Interactions, Vol. 133, No. 1-3, 28.02.2001, p. 159-162.

Research output: Contribution to journalArticle

Colman, RF, Barycki, JJ, Vargo, MA & Wang, J 2001, 'Probing subunit interactions in glutathione S-transferases using affinity labeling', Chemico-Biological Interactions, vol. 133, no. 1-3, pp. 159-162.
Colman, Roberta F. ; Barycki, Joseph J. ; Vargo, Melissa A. ; Wang, Jibo. / Probing subunit interactions in glutathione S-transferases using affinity labeling. In: Chemico-Biological Interactions. 2001 ; Vol. 133, No. 1-3. pp. 159-162.
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