Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine.

K. Negishi, T. Yamashita, W. Zacharias, R. D. Wells, T. Bessho, H. Hayatsu

Research output: Contribution to journalArticle

Abstract

Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions.

Original languageEnglish (US)
Pages (from-to)109-110
Number of pages2
JournalNucleic acids symposium series
Issue number22
StatePublished - 1990

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Cytosine
Salts
DNA
DNA-Directed DNA Polymerase
Nucleic Acids
Plasmids
hydrogen sulfite
methoxyamine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Negishi, K., Yamashita, T., Zacharias, W., Wells, R. D., Bessho, T., & Hayatsu, H. (1990). Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine. Nucleic acids symposium series, (22), 109-110.

Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine. / Negishi, K.; Yamashita, T.; Zacharias, W.; Wells, R. D.; Bessho, T.; Hayatsu, H.

In: Nucleic acids symposium series, No. 22, 1990, p. 109-110.

Research output: Contribution to journalArticle

Negishi, K, Yamashita, T, Zacharias, W, Wells, RD, Bessho, T & Hayatsu, H 1990, 'Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine.', Nucleic acids symposium series, no. 22, pp. 109-110.
Negishi, K. ; Yamashita, T. ; Zacharias, W. ; Wells, R. D. ; Bessho, T. ; Hayatsu, H. / Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine. In: Nucleic acids symposium series. 1990 ; No. 22. pp. 109-110.
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