Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR

O. C. Andronesi, J. R. Pfeifer, L. Al-Momani, S. Özdirekcan, D. T.S. Rijkers, B. Angerstein, S. Luca, U. Koert, J. A. Killian, M. Baldus

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

One and two-dimensional solid-state NMR experiments are discussed that permit probing local structure and overall molecular conformation of membrane-embedded polypeptides under Magic Angle Spinning. The functional dependence of a series of anisotropic recoupling schemes is analyzed using theoretical and numerical methods. These studies lead to the construction of a set of polarization dephasing or transfer units that probe local backbone conformation and overall molecular orientation within the same NMR experiment. Experimental results are shown for a randomly oriented peptide and for two model membrane peptides reconstituted into lipid bilayers and oriented on polymer films according to a method proposed by Bechinger et al.

Original languageEnglish (US)
Pages (from-to)253-265
Number of pages13
JournalJournal of Biomolecular NMR
Volume30
Issue number3
DOIs
StatePublished - Nov 1 2004

Fingerprint

Magic angle spinning
Molecular Conformation
Membrane Proteins
Nuclear magnetic resonance
Peptides
Conformations
Membranes
Lipid bilayers
Molecular orientation
Lipid Bilayers
Polymer films
Numerical methods
Polymers
Experiments
Polarization

Keywords

  • MAS
  • Membrane proteins
  • NMR
  • Solid-state
  • Structure determination

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

Cite this

Andronesi, O. C., Pfeifer, J. R., Al-Momani, L., Özdirekcan, S., Rijkers, D. T. S., Angerstein, B., ... Baldus, M. (2004). Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR. Journal of Biomolecular NMR, 30(3), 253-265. https://doi.org/10.1007/s10858-004-3452-3

Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR. / Andronesi, O. C.; Pfeifer, J. R.; Al-Momani, L.; Özdirekcan, S.; Rijkers, D. T.S.; Angerstein, B.; Luca, S.; Koert, U.; Killian, J. A.; Baldus, M.

In: Journal of Biomolecular NMR, Vol. 30, No. 3, 01.11.2004, p. 253-265.

Research output: Contribution to journalArticle

Andronesi, OC, Pfeifer, JR, Al-Momani, L, Özdirekcan, S, Rijkers, DTS, Angerstein, B, Luca, S, Koert, U, Killian, JA & Baldus, M 2004, 'Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR', Journal of Biomolecular NMR, vol. 30, no. 3, pp. 253-265. https://doi.org/10.1007/s10858-004-3452-3
Andronesi OC, Pfeifer JR, Al-Momani L, Özdirekcan S, Rijkers DTS, Angerstein B et al. Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR. Journal of Biomolecular NMR. 2004 Nov 1;30(3):253-265. https://doi.org/10.1007/s10858-004-3452-3
Andronesi, O. C. ; Pfeifer, J. R. ; Al-Momani, L. ; Özdirekcan, S. ; Rijkers, D. T.S. ; Angerstein, B. ; Luca, S. ; Koert, U. ; Killian, J. A. ; Baldus, M. / Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR. In: Journal of Biomolecular NMR. 2004 ; Vol. 30, No. 3. pp. 253-265.
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