Pristane induced gene activation

Lenora R. Garrett, Clinton J. Jones, Marvin A. Cuchens

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.

Original languageEnglish (US)
Pages (from-to)119-130
Number of pages12
JournalChemico-Biological Interactions
Volume81
Issue number1-2
DOIs
StatePublished - Jan 1992

Fingerprint

Transcriptional Activation
Genes
Tetradecanoylphorbol Acetate
Chemical activation
Acetates
COS Cells
Regulator Genes
pristane
Gene expression
Carcinogens
Plasmids
Cells
Gene Expression
Cell Line

Keywords

  • 12-O-tetradecanoylphorbol 13-acetate
  • Chloramphenicol acetyltransferase
  • Pristane
  • Transfection
  • trans-Activation

ASJC Scopus subject areas

  • Toxicology

Cite this

Garrett, L. R., Jones, C. J., & Cuchens, M. A. (1992). Pristane induced gene activation. Chemico-Biological Interactions, 81(1-2), 119-130. https://doi.org/10.1016/0009-2797(92)90030-O

Pristane induced gene activation. / Garrett, Lenora R.; Jones, Clinton J.; Cuchens, Marvin A.

In: Chemico-Biological Interactions, Vol. 81, No. 1-2, 01.1992, p. 119-130.

Research output: Contribution to journalArticle

Garrett, LR, Jones, CJ & Cuchens, MA 1992, 'Pristane induced gene activation', Chemico-Biological Interactions, vol. 81, no. 1-2, pp. 119-130. https://doi.org/10.1016/0009-2797(92)90030-O
Garrett, Lenora R. ; Jones, Clinton J. ; Cuchens, Marvin A. / Pristane induced gene activation. In: Chemico-Biological Interactions. 1992 ; Vol. 81, No. 1-2. pp. 119-130.
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