Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain

Joshua H. Wong, David B. Marx, Jeff D. Wilson, Bob B. Buchanan, Peggy G. Lemaux, Jeffrey F. Pedersen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Limited progress has been made on genetic improvement of the digestibility of sorghum grain because of variability among different varieties. In this study, we applied multiple techniques to assess digestibility of grain from 18 sorghum lines to identify major components responsible for variability. We also identified storage proteins and enzymes as potential targets for genetic modification to improve digestibility. Results from principal component analysis revealed that content of amylose and total starch, together with protein digestibility (PD), accounted for 94% of variation in digestibility. Control of amylose content is understood and manageable. Up-regulation of genes associated with starch accumulation is clearly a future target for improving digestibility. To identify proteins that might be targets for future modification, meal from selected lines was digested in vitro with pancreatin in parallel with pepsin and α-amylase. The %PD was influenced by both the nature of the protein matrix and protein body packaging. Owing to its ability to form oligomers, the 20 kDa γ-kafirin was more resistant to digestion than counterparts lacking this ability, making it a target for down-regulation. Greater understanding of interactions among the three traits identified by principal component analysis is needed for both waxy and non-waxy varieties.

Original languageEnglish (US)
Pages (from-to)598-611
Number of pages14
JournalPlant Science
Volume179
Issue number6
DOIs
StatePublished - Dec 1 2010

Fingerprint

Sorghum
grain sorghum
Principal Component Analysis
Starch
principal component analysis
digestibility
starch
Amylose
Proteins
digestible protein
amylose
proteins
kafirins
pancreatin
Pancreatin
protein bodies
pepsin
storage proteins
genetic engineering
amylases

Keywords

  • Disulfide proteins
  • Granule-bound starch synthase I
  • Principal component analysis
  • Sorghum
  • Starch-protein interface

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain. / Wong, Joshua H.; Marx, David B.; Wilson, Jeff D.; Buchanan, Bob B.; Lemaux, Peggy G.; Pedersen, Jeffrey F.

In: Plant Science, Vol. 179, No. 6, 01.12.2010, p. 598-611.

Research output: Contribution to journalArticle

Wong, Joshua H. ; Marx, David B. ; Wilson, Jeff D. ; Buchanan, Bob B. ; Lemaux, Peggy G. ; Pedersen, Jeffrey F. / Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain. In: Plant Science. 2010 ; Vol. 179, No. 6. pp. 598-611.
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