Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile

Erika Van Eijk, Vasileios Paschalis, Matthew Green, Annemieke H. Friggen, Marilynn A. Larson, Keith Spriggs, Geoffrey S. Briggs, Panos Soultanas, Wiep Klaas Smits

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA replication in pathogenic microorganisms. Clostridium difficile is the main cause of healthcare-associated diarrhoea and its DNA replication machinery is virtually uncharacterized. We identify and characterize the mechanistic details of the putative replicative helicase (CD3657), helicase-loader ATPase (CD3654) and primase (CD1454) of C. difficile, and reconstitute helicase and primase activities in vitro. We demonstrate a direct and ATP-dependent interaction between the helicase loader and the helicase. Furthermore, we find that helicase activity is dependent on the presence of primase in vitro. The inherent trinucleotide specificity of primase is determined by a single lysine residue and is similar to the primase of the extreme thermophile Aquifex aeolicus. However, the presence of helicase allows more efficient de novo synthesis of RNA primers from non-preferred trinucleotides. Thus, loader-helicase-primase interactions, which crucially mediate helicase loading and activation during DNA replication in all organisms, differ critically in C. difficile from that of the well-studied Gram-positive Bacillus subtilis model.

Original languageEnglish (US)
Article number160272
JournalOpen Biology
Volume6
Issue number12
DOIs
StatePublished - Jan 1 2016

Fingerprint

DNA Primase
Clostridium
Clostridium difficile
DNA Replication
Loaders
DNA
Bacilli
Bacillus subtilis
Microorganisms
Lysine
Machinery
Adenosine Triphosphatases
Diarrhea
Adenosine Triphosphate
Chemical activation
Delivery of Health Care

Keywords

  • ATPase
  • Clostridium difficile
  • DNA replication initiation
  • Helicase loading and activation
  • Primase trinucleotide specificity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile. / Van Eijk, Erika; Paschalis, Vasileios; Green, Matthew; Friggen, Annemieke H.; Larson, Marilynn A.; Spriggs, Keith; Briggs, Geoffrey S.; Soultanas, Panos; Smits, Wiep Klaas.

In: Open Biology, Vol. 6, No. 12, 160272, 01.01.2016.

Research output: Contribution to journalArticle

Van Eijk, E, Paschalis, V, Green, M, Friggen, AH, Larson, MA, Spriggs, K, Briggs, GS, Soultanas, P & Smits, WK 2016, 'Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile', Open Biology, vol. 6, no. 12, 160272. https://doi.org/10.1098/rsob.160272
Van Eijk, Erika ; Paschalis, Vasileios ; Green, Matthew ; Friggen, Annemieke H. ; Larson, Marilynn A. ; Spriggs, Keith ; Briggs, Geoffrey S. ; Soultanas, Panos ; Smits, Wiep Klaas. / Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile. In: Open Biology. 2016 ; Vol. 6, No. 12.
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