Primary structures and biological activities of substance‐P‐related peptides from the brain of the dogfish, Scyliorhinus canicula

David WAUGH, Yunxia WANG, Neil HAZON, Richard J. BALMENT, J. Michael CONLON

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two peptides with substance‐P‐like immunoreactivity were isolated in pure form from an extract of the brain of the elasmobranch fish, Scyliorhinus canicula (european common dogfish). One peptide was identical to scyliorhinin I, previously identified in dogfish intestine, and the second was the undecapeptide Lys‐Pro‐Arg‐Pro‐Gly‐Gln‐Phe‐Phe‐Gly‐Leu‐Met‐CONH2 which is structurally similar to mammalian substance P. Scyliorhinin II or a peptide analogous to mammalian neurokinin A were not detected in the extract. Synthetic dogfish substance P ([Lys1, Arg3, Gly5]substance P) was approximately threefold more potent than mammalian substance P (Kd= 0.21±0.11 nM versus Kd= 0.74±0.17 nM; mean ± SD; n= 6) in inhibiting the binding of 125I‐labelled substance P to neurokinin (NK1) receptors in rat submandibular gland membranes. The vasodilator action of tachykinins in mammals is mediated primarily through interaction with NK1 receptors. Bolus intravenous injections of [Lys1, Arg3, Gly5]substance P (100 pmol) and scyliorhinin I (100 pmol) produced appreciable (>4kPa) decreases in arterial blood pressure in the rat whereas intravenous injections of up to 5 nmol of the peptides into conscious, unrestrained dogfish produced no change in arterial blood pressure, pulse amplitude or heart rate. Injections of greater amounts of the peptides (10–50 nmol) produced a slight increase (400–667 Pa) in blood pressure. The data indicate that mammalian‐type NK1 tachykinin receptors are not involved in cardiovascular regulation in elasmobranch fish.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
JournalEuropean Journal of Biochemistry
Volume214
Issue number2
DOIs
StatePublished - Jun 1993

Fingerprint

Dogfish
Substance P
Bioactivity
Brain
Peptides
Blood pressure
Elasmobranchii
Intravenous Injections
Fish
Rats
Arterial Pressure
Fishes
Heart Rate
Tachykinin Receptors
Neurokinin A
Tachykinins
Mammals
Submandibular Gland
Vasodilator Agents
Intestines

ASJC Scopus subject areas

  • Biochemistry

Cite this

Primary structures and biological activities of substance‐P‐related peptides from the brain of the dogfish, Scyliorhinus canicula. / WAUGH, David; WANG, Yunxia; HAZON, Neil; BALMENT, Richard J.; CONLON, J. Michael.

In: European Journal of Biochemistry, Vol. 214, No. 2, 06.1993, p. 469-474.

Research output: Contribution to journalArticle

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abstract = "Two peptides with substance‐P‐like immunoreactivity were isolated in pure form from an extract of the brain of the elasmobranch fish, Scyliorhinus canicula (european common dogfish). One peptide was identical to scyliorhinin I, previously identified in dogfish intestine, and the second was the undecapeptide Lys‐Pro‐Arg‐Pro‐Gly‐Gln‐Phe‐Phe‐Gly‐Leu‐Met‐CONH2 which is structurally similar to mammalian substance P. Scyliorhinin II or a peptide analogous to mammalian neurokinin A were not detected in the extract. Synthetic dogfish substance P ([Lys1, Arg3, Gly5]substance P) was approximately threefold more potent than mammalian substance P (Kd= 0.21±0.11 nM versus Kd= 0.74±0.17 nM; mean ± SD; n= 6) in inhibiting the binding of 125I‐labelled substance P to neurokinin (NK1) receptors in rat submandibular gland membranes. The vasodilator action of tachykinins in mammals is mediated primarily through interaction with NK1 receptors. Bolus intravenous injections of [Lys1, Arg3, Gly5]substance P (100 pmol) and scyliorhinin I (100 pmol) produced appreciable (>4kPa) decreases in arterial blood pressure in the rat whereas intravenous injections of up to 5 nmol of the peptides into conscious, unrestrained dogfish produced no change in arterial blood pressure, pulse amplitude or heart rate. Injections of greater amounts of the peptides (10–50 nmol) produced a slight increase (400–667 Pa) in blood pressure. The data indicate that mammalian‐type NK1 tachykinin receptors are not involved in cardiovascular regulation in elasmobranch fish.",
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