Primary structure of goat myoglobin

S. P. Suman, P. Joseph, S. Li, Laurey A Steinke, M. Fontaine

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Color stability attributes of goat meat are different from those of sheep meat, possibly due to species-specific differences in myoglobin (Mb) biochemistry. An examination of post-genomic era protein databases revealed that the primary structure of goat Mb has not been determined. Therefore, our objective was to characterize the primary structure of goat Mb. Goat Mb was isolated from cardiac muscles employing ammonium sulfate precipitation and gel-filtration chromatography, and Edman degradation was utilized to determine the amino acid sequence. Sequence analyses of intact Mb as well as tryptic- and cyanogen bromide-peptides yielded the complete primary structure of goat Mb, which shared 98.7% similarity with sheep Mb. Similar to other livestock myoglobins goat Mb has 153 residues. Comparison of the sequences of goat and sheep myoglobins revealed two amino acid substitutions - THRgoat8GLNsheep and GLYgoat52GLUsheep. Goat Mb contains 12 histidine residues. As observed in other meat-producing livestock species, distal and proximal histidines, responsible for stabilizing the heme group and coordinating oxygen-binding, are conserved in goat Mb.

Original languageEnglish (US)
Pages (from-to)456-460
Number of pages5
JournalMeat Science
Volume82
Issue number4
DOIs
StatePublished - Aug 1 2009

Fingerprint

myoglobin
Myoglobin
Goats
goats
Meat
Sheep
Livestock
histidine
Histidine
livestock
cyanogen
sheep meat
goat meat
sheep
Cyanogen Bromide
Protein Databases
Ammonium Sulfate
amino acid substitution
heme
Amino Acid Substitution

Keywords

  • Capra hircus
  • Edman degradation
  • Goat meat
  • Mass spectrometry
  • Meat color
  • Myoglobin
  • Primary structure

ASJC Scopus subject areas

  • Food Science

Cite this

Suman, S. P., Joseph, P., Li, S., Steinke, L. A., & Fontaine, M. (2009). Primary structure of goat myoglobin. Meat Science, 82(4), 456-460. https://doi.org/10.1016/j.meatsci.2009.02.017

Primary structure of goat myoglobin. / Suman, S. P.; Joseph, P.; Li, S.; Steinke, Laurey A; Fontaine, M.

In: Meat Science, Vol. 82, No. 4, 01.08.2009, p. 456-460.

Research output: Contribution to journalArticle

Suman, SP, Joseph, P, Li, S, Steinke, LA & Fontaine, M 2009, 'Primary structure of goat myoglobin', Meat Science, vol. 82, no. 4, pp. 456-460. https://doi.org/10.1016/j.meatsci.2009.02.017
Suman, S. P. ; Joseph, P. ; Li, S. ; Steinke, Laurey A ; Fontaine, M. / Primary structure of goat myoglobin. In: Meat Science. 2009 ; Vol. 82, No. 4. pp. 456-460.
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