Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut

Yunxia W Lundberg, T. Badgery-Parker, S. Lovas, N. Chartrel, H. Vaudry, E. Burcher, J. M. Conlon

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Abstract

A tachykinin peptide was isolated from an extract of the intestine of the European green frog, Rana ridibunda, and its primary structure was established as:His-Lys-Leu-Asp-Ser-phe-Ile-Gly-Leu-Met.CONH2. This sequence was confirmed by chemical synthesis and shows two amino acid substitutions (leucine for threonine at position 3 and isoleucine for valine at position 7) compared with neurokinin A. Binding parameters for synthetic [Leu3,Ile7]neurokinin A and mammalian tachykinins were compared using receptor-selective radioligands and crude membranes from tissues enriched in the NK1, NK2 and NK3 receptors. [Leu3,Ile7]Neurokinin A was approx. 3-fold less potent than substance P in inhibiting the binding of 125I-labelled [Sar9,Met(O2)11]substance P (labelled with Bolton-Hunter reagent) to rat submandibular gland (NK1 receptor), 8-fold less potent than neurokinin A in inhibiting the binding of [2-[125I]iodohistidine(l)]neurokinin A to rat stomach fundus (NK2 receptor) and 6-fold less potent than neurokinin B in inhibiting the binding of 125I-Bolton-Hunter-labelled scyliorhinin II to rat brain (NK3 receptor). Thus the frog neurokinin A-related peptide shows moderate affinity but lack of selectively for all three tachykinin-binding sites in rat tissues. This non-selectivity is similar to that displayed by the molluscan tachykinin, eledoisin, which also contains an isoleucine residue in the corresponding position in the molecule.

Original languageEnglish (US)
Pages (from-to)827-832
Number of pages6
JournalBiochemical Journal
Volume287
Issue number3
DOIs
StatePublished - Jan 1 1992

Fingerprint

Neurokinin A
Anura
Tachykinins
Peptides
Rats
Isoleucine
Substance P
Rana clamitans
Eledoisin
Rana ridibunda
Neurokinin B
Tissue
Submandibular Gland
Valine
Threonine
Amino Acid Substitution
Leucine
Intestines
Brain
Stomach

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lundberg, Y. W., Badgery-Parker, T., Lovas, S., Chartrel, N., Vaudry, H., Burcher, E., & Conlon, J. M. (1992). Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut. Biochemical Journal, 287(3), 827-832. https://doi.org/10.1042/bj2870827

Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut. / Lundberg, Yunxia W; Badgery-Parker, T.; Lovas, S.; Chartrel, N.; Vaudry, H.; Burcher, E.; Conlon, J. M.

In: Biochemical Journal, Vol. 287, No. 3, 01.01.1992, p. 827-832.

Research output: Contribution to journalArticle

Lundberg, YW, Badgery-Parker, T, Lovas, S, Chartrel, N, Vaudry, H, Burcher, E & Conlon, JM 1992, 'Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut', Biochemical Journal, vol. 287, no. 3, pp. 827-832. https://doi.org/10.1042/bj2870827
Lundberg, Yunxia W ; Badgery-Parker, T. ; Lovas, S. ; Chartrel, N. ; Vaudry, H. ; Burcher, E. ; Conlon, J. M. / Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut. In: Biochemical Journal. 1992 ; Vol. 287, No. 3. pp. 827-832.
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