Primary cilia are decreased in breast cancer: Analysis of a collection of human breast cancer cell lines and tissues

Kun Yuan, Natalya Frolova, Yi Xie, Dezhi Wang, Leah Cook, Yeon Jin Kwon, Adam D. Steg, Rosa Serra, Andra R. Frost

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Primary cilia (PC) are solitary, sensory organelles that are critical for several signaling pathways. PC were detected by immunofluorescence of cultured cells and breast tissues. After growth for 7 days in vitro, PC were detected in ∼70% of breast fibroblasts and in 7-19% of epithelial cells derived from benign breast (184A1 and MCF10A). In 11 breast cancer cell lines, PC were present at a low frequency in four (from 0.3% to 4% of cells), but were absent in the remainder. The cancer cell lines with PC were all of the basal B subtype, which is analogous to the clinical triple-negative breast cancer subtype. Furthermore, the frequency of PC decreased with increasing degree of transformation/progression in the MCF10 and MDA-MB-435/LCC6 isogenic models of cancer progression. In histologically normal breast tissues, PC were frequent in fibroblasts and myoepithelial cells and less common in luminal epithelial cells. Of 26 breast cancers examined, rare PC were identified in cancer epithelial cells of only one cancer, which was of the triple-negative subtype. These data indicate a decrease or loss of PC in breast cancer and an association of PC with the basal B subtype. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

Original languageEnglish (US)
Pages (from-to)857-870
Number of pages14
JournalJournal of Histochemistry and Cytochemistry
Volume58
Issue number10
DOIs
StatePublished - Oct 2010

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Keywords

  • Breast cancer
  • Carcinogenesis
  • Ki-67
  • Primary cilia
  • Tubulin

ASJC Scopus subject areas

  • Anatomy
  • Histology

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