Primaquine-Induced Hemolytic Anemia: Susceptibility of Normal versus Glutathione-Depleted Rat Erythrocytes to 5-Hydroxyprimaquine

Zachary S. Bowman, John E. Oatis, Jennifer L. Whelan, David J. Jollow, David C. McMillan

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56 Citations (Scopus)

Abstract

Primaquine is an important antimalarial agent because of its activity against exoerythrocytic forms of Plasmodium spp. Methemoglobinemia and hemolytic anemia, however, are dose-limiting side effects of primaquine therapy. These hemotoxic effects are believed to be mediated by metabolites, although the identity of the toxic specie(s) and the mechanism underlying hemotoxicity have remained unclear. Previous studies showed that an N-hydroxylated metabolite of primaquine, 6-methoxy-8-hydroxylaminoquinoline, was capable of mediating primaquine-induced hemotoxicity. The present studies were undertaken to investigate the hemolytic potential of 5-hydroxyprimaquine (5-HPQ), a phenolic metabolite that has been detected in experimental animals. 5-HPQ was synthesized, isolated by flash chromatography, and characterized by NMR spectroscopy and mass spectrometry. In vitro exposure of 51Cr-labeled erythrocytes to 5-HPQ induced a concentration-dependent decrease in erythrocyte survival (TC50 of ca. 40 μM) when the exposed cells were returned to the circulation of isologous rats. 5-HPQ also induced methemoglobin formation and depletion of glutathione (GSH) when incubated with suspensions of rat erythrocytes. Furthermore, when red cell GSH was depleted (>95%) by titration with diethyl maleate to mimic GSH instability in human glucose-6-phosphate dehydrogenase deficiency, a 5-fold enhancement of hemolytic activity was observed. These data indicate that 5-HPQ also has the requisite properties to contribute to the hemotoxicity of primaquine. The relative contribution of N-hydroxy versus phenolic metabolites to the overall hemotoxicity of primaquine remains to be assessed.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume309
Issue number1
DOIs
StatePublished - Apr 1 2004

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Primaquine
Hemolytic Anemia
Glutathione
Erythrocytes
diethyl maleate
Erythrocyte Aging
Methemoglobinemia
Glucosephosphate Dehydrogenase Deficiency
Methemoglobin
Plasmodium
Poisons
Antimalarials
5-hydroxyprimaquine
Chromatography
Mass Spectrometry
Suspensions
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Pharmacology

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Primaquine-Induced Hemolytic Anemia : Susceptibility of Normal versus Glutathione-Depleted Rat Erythrocytes to 5-Hydroxyprimaquine. / Bowman, Zachary S.; Oatis, John E.; Whelan, Jennifer L.; Jollow, David J.; McMillan, David C.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 309, No. 1, 01.04.2004, p. 79-85.

Research output: Contribution to journalArticle

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