Prevention of Pericyte Ghost Formation in Retinal Capillaries of Galactose-Fed Dogs by Aldose Reductase Inhibitors

P. F. Kador, Y. Akagi, H. Terubayashi, M. Wyman, J. H. Kinoshita

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A distinguishing feature of early diabetic retinal vascular changes is the selective degeneration of pericytes (mural cells) from the retinal capillary vessels. Loss of these pericytes has been proposed to be associated with decreased capillary tonicity, the formation of microaneurysms, and vessel dilation. The role of aldose reductase in the progression of diabetic retinopathy has been investigated in age- and sex-matched beagles fed a 30% galactose diet with or without the aldose reductase inhibitors sorbinil or M79175. Eyes were periodically enucleated from dogs in each group and their retinal capillaries were examined as trypsin-digested flat preparations. Before the clinical appearance of retinal changes, pericyte ghost formation was observed in the eyes of three fourths of the dogs after 21 months and all of the dogs after 24 months of galactose feeding. Many of the capillaries containing pericyte ghosts demonstrated an apparent proliferation of endothelial cells and acellular vessels. No pericyte ghosts or abnormal findings were observed in retinas from either normal control (zero of nine) or galactose-fed dogs treated with aldose reductase inhibitors (zero of 16). These findings indicate that aldose reductase inhibitors can prevent the formation of pericyte ghosts and other subsequent capillary changes in experimental retinopathy.

Original languageEnglish (US)
Pages (from-to)1099-1102
Number of pages4
JournalArchives of Ophthalmology
Volume106
Issue number8
DOIs
StatePublished - Aug 1988

Fingerprint

Aldehyde Reductase
Pericytes
Galactose
Dogs
Retinal Vessels
Diabetic Retinopathy
Trypsin
Retina
Dilatation
Endothelial Cells
Diet

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Prevention of Pericyte Ghost Formation in Retinal Capillaries of Galactose-Fed Dogs by Aldose Reductase Inhibitors. / Kador, P. F.; Akagi, Y.; Terubayashi, H.; Wyman, M.; Kinoshita, J. H.

In: Archives of Ophthalmology, Vol. 106, No. 8, 08.1988, p. 1099-1102.

Research output: Contribution to journalArticle

Kador, P. F. ; Akagi, Y. ; Terubayashi, H. ; Wyman, M. ; Kinoshita, J. H. / Prevention of Pericyte Ghost Formation in Retinal Capillaries of Galactose-Fed Dogs by Aldose Reductase Inhibitors. In: Archives of Ophthalmology. 1988 ; Vol. 106, No. 8. pp. 1099-1102.
@article{a70935d5bad44026a444b3164e96788d,
title = "Prevention of Pericyte Ghost Formation in Retinal Capillaries of Galactose-Fed Dogs by Aldose Reductase Inhibitors",
abstract = "A distinguishing feature of early diabetic retinal vascular changes is the selective degeneration of pericytes (mural cells) from the retinal capillary vessels. Loss of these pericytes has been proposed to be associated with decreased capillary tonicity, the formation of microaneurysms, and vessel dilation. The role of aldose reductase in the progression of diabetic retinopathy has been investigated in age- and sex-matched beagles fed a 30{\%} galactose diet with or without the aldose reductase inhibitors sorbinil or M79175. Eyes were periodically enucleated from dogs in each group and their retinal capillaries were examined as trypsin-digested flat preparations. Before the clinical appearance of retinal changes, pericyte ghost formation was observed in the eyes of three fourths of the dogs after 21 months and all of the dogs after 24 months of galactose feeding. Many of the capillaries containing pericyte ghosts demonstrated an apparent proliferation of endothelial cells and acellular vessels. No pericyte ghosts or abnormal findings were observed in retinas from either normal control (zero of nine) or galactose-fed dogs treated with aldose reductase inhibitors (zero of 16). These findings indicate that aldose reductase inhibitors can prevent the formation of pericyte ghosts and other subsequent capillary changes in experimental retinopathy.",
author = "Kador, {P. F.} and Y. Akagi and H. Terubayashi and M. Wyman and Kinoshita, {J. H.}",
year = "1988",
month = "8",
doi = "10.1001/archopht.1988.01060140255036",
language = "English (US)",
volume = "106",
pages = "1099--1102",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "8",

}

TY - JOUR

T1 - Prevention of Pericyte Ghost Formation in Retinal Capillaries of Galactose-Fed Dogs by Aldose Reductase Inhibitors

AU - Kador, P. F.

AU - Akagi, Y.

AU - Terubayashi, H.

AU - Wyman, M.

AU - Kinoshita, J. H.

PY - 1988/8

Y1 - 1988/8

N2 - A distinguishing feature of early diabetic retinal vascular changes is the selective degeneration of pericytes (mural cells) from the retinal capillary vessels. Loss of these pericytes has been proposed to be associated with decreased capillary tonicity, the formation of microaneurysms, and vessel dilation. The role of aldose reductase in the progression of diabetic retinopathy has been investigated in age- and sex-matched beagles fed a 30% galactose diet with or without the aldose reductase inhibitors sorbinil or M79175. Eyes were periodically enucleated from dogs in each group and their retinal capillaries were examined as trypsin-digested flat preparations. Before the clinical appearance of retinal changes, pericyte ghost formation was observed in the eyes of three fourths of the dogs after 21 months and all of the dogs after 24 months of galactose feeding. Many of the capillaries containing pericyte ghosts demonstrated an apparent proliferation of endothelial cells and acellular vessels. No pericyte ghosts or abnormal findings were observed in retinas from either normal control (zero of nine) or galactose-fed dogs treated with aldose reductase inhibitors (zero of 16). These findings indicate that aldose reductase inhibitors can prevent the formation of pericyte ghosts and other subsequent capillary changes in experimental retinopathy.

AB - A distinguishing feature of early diabetic retinal vascular changes is the selective degeneration of pericytes (mural cells) from the retinal capillary vessels. Loss of these pericytes has been proposed to be associated with decreased capillary tonicity, the formation of microaneurysms, and vessel dilation. The role of aldose reductase in the progression of diabetic retinopathy has been investigated in age- and sex-matched beagles fed a 30% galactose diet with or without the aldose reductase inhibitors sorbinil or M79175. Eyes were periodically enucleated from dogs in each group and their retinal capillaries were examined as trypsin-digested flat preparations. Before the clinical appearance of retinal changes, pericyte ghost formation was observed in the eyes of three fourths of the dogs after 21 months and all of the dogs after 24 months of galactose feeding. Many of the capillaries containing pericyte ghosts demonstrated an apparent proliferation of endothelial cells and acellular vessels. No pericyte ghosts or abnormal findings were observed in retinas from either normal control (zero of nine) or galactose-fed dogs treated with aldose reductase inhibitors (zero of 16). These findings indicate that aldose reductase inhibitors can prevent the formation of pericyte ghosts and other subsequent capillary changes in experimental retinopathy.

UR - http://www.scopus.com/inward/record.url?scp=0023694725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023694725&partnerID=8YFLogxK

U2 - 10.1001/archopht.1988.01060140255036

DO - 10.1001/archopht.1988.01060140255036

M3 - Article

C2 - 3401138

AN - SCOPUS:0023694725

VL - 106

SP - 1099

EP - 1102

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 8

ER -