Prevention of nerve edema and increased blood-nerve barrier permeability-surface area product in galactosemic rats by aldose reductase or thromboxane synthetase inhibitors

K. C. Wadhwani, L. E. Caspers-Velu, V. A. Murphy, Q. R. Smith, P. F. Kador, S. I. Rapoport

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Nerve water content and the permeability-surface area product (PA) to [3H]- or [14C]sucrose at the blood-nerve barrier were determined in unanesthetized control rats fed a normal diet and in rats fed galactose with or without an aldose reductase inhibitor (Statil or AL 1576) or a thromboxane synthetase inhibitor (CGS 12970). Nerve water content was determined by taking the difference between dry and wet weights of whole tibial nerves. PA was determined by an intravenous bolus injection of radiotracer with multiple-time-point graphic and quantitative autoradiographic methods. The mean nerve water content in galactosemic rats was 15% higher than in control rats after 7-11 mo on the diet. Statil and AL 1576 prevented nerve edema, but CGS 12970 was only partially effective in preventing an increase in nerve water content in galactose-fed rats. In galactosemic rats, the mean PA to sucrose at the blood-nerve barrier, calculated from nerve dry weight, was twofold higher than in control rats. Treatment with Statil, AL 1576, or CGS 12970 prevented increased PA. Our results suggest that nerve edema and increased bood-nerve barrier PA are secondary to polyol production and can be prevented by inhibiting aldose reductase.

Original languageEnglish (US)
Pages (from-to)1469-1477
Number of pages9
JournalDiabetes
Volume38
Issue number11
DOIs
StatePublished - Jan 1 1989

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Blood-Nerve Barrier
Thromboxane-A Synthase
Aldehyde Reductase
Permeability
Edema
Water
Galactose
Sucrose
Diet
Weights and Measures
Tibial Nerve
Intravenous Injections

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Prevention of nerve edema and increased blood-nerve barrier permeability-surface area product in galactosemic rats by aldose reductase or thromboxane synthetase inhibitors. / Wadhwani, K. C.; Caspers-Velu, L. E.; Murphy, V. A.; Smith, Q. R.; Kador, P. F.; Rapoport, S. I.

In: Diabetes, Vol. 38, No. 11, 01.01.1989, p. 1469-1477.

Research output: Contribution to journalArticle

Wadhwani, K. C. ; Caspers-Velu, L. E. ; Murphy, V. A. ; Smith, Q. R. ; Kador, P. F. ; Rapoport, S. I. / Prevention of nerve edema and increased blood-nerve barrier permeability-surface area product in galactosemic rats by aldose reductase or thromboxane synthetase inhibitors. In: Diabetes. 1989 ; Vol. 38, No. 11. pp. 1469-1477.
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