Prevalence of shiga-toxigenic Escherichia coli O157:H7 in adult dairy cattle

John R. Dunn, James E. Keen, R. Alex Thompson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective - To describe shiga-toxigenic Escherichia coli O157:H7 (STEC O157:H7) fecal shedding prevalence, seasonal fecal shedding patterns, and site-specific prevalence from the oral cavity, skin, and feces of dairy cattle. Design - Cross-sectional study. Animals-Adult dairy cattle from 13 herds in Louisiana. Procedure - Samples were cultured for STEC O157 by use of sensitive and specific techniques, including selective broth enrichment, immunomagnetic separation, monoclonal antibody-based O:H enzyme immunoassay serotyping, and polymerase chain reaction virulence gene characterization. Point estimates and 95% confidence intervals were calculated for fecal shedding prevalence as well as site-specific prevalence from the oral cavity, skin, and feces. Logistic regression was used to assess seasonal variation and differences at various stages of lactation with respect to fecal shedding of STEC O157 in cattle sampled longitudinally. Results - Summer prevalence in herds (n = 13) was 38.5%, with a cow-level prevalence of 6.5%. Among positive herds, prevalence ranged from 3% to 34.6%. Samples from 3 of 5 herds sampled quarterly over 1 year yielded positive results for STEC O157. In herds with STEC O157, an increase in cow-level prevalence was detected during spring (13.3%) and summer (10,5%), compared with values for fall and winter. Site-specific prevalences of STEC O157:H7 from oral cavity, skin, and fecal samples were 0%, 0.7%, and 25.2%, respectively. Conclusions and Clinical Relevance - Our data indicated that STEC O157:H7 was commonly isolated from dairy cows in Louisiana, seasonally shed, and isolated from the skin surface but not the oral cavity of cows.

Original languageEnglish (US)
Pages (from-to)1151-1158
Number of pages8
JournalJournal of the American Veterinary Medical Association
Volume224
Issue number7
DOIs
StatePublished - Apr 1 2004

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Shiga-Toxigenic Escherichia coli
Shiga toxin-producing Escherichia coli
Escherichia coli O157
dairy cattle
skin (animal)
herds
Mouth
mouth
Skin
cows
Feces
feces
Immunomagnetic Separation
immunomagnetic separation
Serotyping
summer
enzyme immunoassays
lactation stage
Immunoenzyme Techniques
Lactation

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Prevalence of shiga-toxigenic Escherichia coli O157:H7 in adult dairy cattle. / Dunn, John R.; Keen, James E.; Thompson, R. Alex.

In: Journal of the American Veterinary Medical Association, Vol. 224, No. 7, 01.04.2004, p. 1151-1158.

Research output: Contribution to journalArticle

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abstract = "Objective - To describe shiga-toxigenic Escherichia coli O157:H7 (STEC O157:H7) fecal shedding prevalence, seasonal fecal shedding patterns, and site-specific prevalence from the oral cavity, skin, and feces of dairy cattle. Design - Cross-sectional study. Animals-Adult dairy cattle from 13 herds in Louisiana. Procedure - Samples were cultured for STEC O157 by use of sensitive and specific techniques, including selective broth enrichment, immunomagnetic separation, monoclonal antibody-based O:H enzyme immunoassay serotyping, and polymerase chain reaction virulence gene characterization. Point estimates and 95{\%} confidence intervals were calculated for fecal shedding prevalence as well as site-specific prevalence from the oral cavity, skin, and feces. Logistic regression was used to assess seasonal variation and differences at various stages of lactation with respect to fecal shedding of STEC O157 in cattle sampled longitudinally. Results - Summer prevalence in herds (n = 13) was 38.5{\%}, with a cow-level prevalence of 6.5{\%}. Among positive herds, prevalence ranged from 3{\%} to 34.6{\%}. Samples from 3 of 5 herds sampled quarterly over 1 year yielded positive results for STEC O157. In herds with STEC O157, an increase in cow-level prevalence was detected during spring (13.3{\%}) and summer (10,5{\%}), compared with values for fall and winter. Site-specific prevalences of STEC O157:H7 from oral cavity, skin, and fecal samples were 0{\%}, 0.7{\%}, and 25.2{\%}, respectively. Conclusions and Clinical Relevance - Our data indicated that STEC O157:H7 was commonly isolated from dairy cows in Louisiana, seasonally shed, and isolated from the skin surface but not the oral cavity of cows.",
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