Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

Paul D Fey, R. S. Wickert, Mark Edmund Rupp, T. J. Safranek, Steven Heye Hinrichs

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.

Original languageEnglish (US)
Pages (from-to)530-533
Number of pages4
JournalEmerging infectious diseases
Volume6
Issue number5
DOIs
StatePublished - Jan 1 2000

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Cefixime
Enterohemorrhagic Escherichia coli
Shiga-Toxigenic Escherichia coli
Sorbitol
Immunoenzyme Techniques
Polymerase Chain Reaction
Serogroup
tellurous acid

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska. / Fey, Paul D; Wickert, R. S.; Rupp, Mark Edmund; Safranek, T. J.; Hinrichs, Steven Heye.

In: Emerging infectious diseases, Vol. 6, No. 5, 01.01.2000, p. 530-533.

Research output: Contribution to journalArticle

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