Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa

Ellen C. Jensen, Holly S. Schrader, Brenda Rieland, Thomas L. Thompson, Kit W. Lee, Kenneth W. Nickerson, Tyler A. Kokjohn

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.

Original languageEnglish (US)
Pages (from-to)575-580
Number of pages6
JournalApplied and environmental microbiology
Volume64
Issue number2
StatePublished - Feb 1 1998

Fingerprint

Sphaerotilus
Sphaerotilus natans
Host Specificity
host range
bacteriophage
Pseudomonas aeruginosa
bacteriophages
Bacteriophages
Escherichia coli
Type I Site Specific Deoxyribonucleases
Type II Site Specific Deoxyribonucleases
gene transfer
sewage treatment
cleavage
Selection Bias
restriction endonucleases
Sewage
Ecology

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Jensen, E. C., Schrader, H. S., Rieland, B., Thompson, T. L., Lee, K. W., Nickerson, K. W., & Kokjohn, T. A. (1998). Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa. Applied and environmental microbiology, 64(2), 575-580.

Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa. / Jensen, Ellen C.; Schrader, Holly S.; Rieland, Brenda; Thompson, Thomas L.; Lee, Kit W.; Nickerson, Kenneth W.; Kokjohn, Tyler A.

In: Applied and environmental microbiology, Vol. 64, No. 2, 01.02.1998, p. 575-580.

Research output: Contribution to journalArticle

Jensen, Ellen C. ; Schrader, Holly S. ; Rieland, Brenda ; Thompson, Thomas L. ; Lee, Kit W. ; Nickerson, Kenneth W. ; Kokjohn, Tyler A. / Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa. In: Applied and environmental microbiology. 1998 ; Vol. 64, No. 2. pp. 575-580.
@article{e60b253e17394013bce4a0fcc960865c,
title = "Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa",
abstract = "Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.",
author = "Jensen, {Ellen C.} and Schrader, {Holly S.} and Brenda Rieland and Thompson, {Thomas L.} and Lee, {Kit W.} and Nickerson, {Kenneth W.} and Kokjohn, {Tyler A.}",
year = "1998",
month = "2",
day = "1",
language = "English (US)",
volume = "64",
pages = "575--580",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa

AU - Jensen, Ellen C.

AU - Schrader, Holly S.

AU - Rieland, Brenda

AU - Thompson, Thomas L.

AU - Lee, Kit W.

AU - Nickerson, Kenneth W.

AU - Kokjohn, Tyler A.

PY - 1998/2/1

Y1 - 1998/2/1

N2 - Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.

AB - Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.

UR - http://www.scopus.com/inward/record.url?scp=0031912436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031912436&partnerID=8YFLogxK

M3 - Article

C2 - 9464396

AN - SCOPUS:0031912436

VL - 64

SP - 575

EP - 580

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -