Presentation of a foreign peptide on the surface of tomato bushy stunt virus

Thorleif Joelson, Lennart Åkerblom, Per Oxelfelt, Bror Strandberg, Karin Tomenius, T. Jack Morris

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A 13-amino-acid peptide derived from the V3 loop of human immunodeficiency virus (HIV-1) glycoprotein 120 (gp120) was attached as a C-terminal gene fusion to the coat protein of tomato bushy stunt virus (TBSV). The architecture of this plant virus permitted external display of the foreign sequence 180 times on the surface of the chimaeric virus particle. The chimaera replicated to a level similar to wild-type TBSV and the foreign sequence was retained through six sequential passages in plants. The HIV epitope was detected on the surface of the virus capsid by a VS-specific monoclonal antibody and by human sera from HIV-1-positive patients, demonstrating the potential of using plant-derived chimaeric particles for diagnostic purposes. Chimaeric virus also induced a specific immune response to the foreign HIV epitope when injected into NMRI mice.

Original languageEnglish (US)
Pages (from-to)1213-1217
Number of pages5
JournalJournal of General Virology
Volume78
Issue number6
DOIs
StatePublished - Jun 1997

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Tombusvirus
HIV-1
Peptides
Epitopes
HIV
Viruses
Plant Viruses
Capsid
Gene Fusion
Capsid Proteins
Virion
Glycoproteins
Monoclonal Antibodies
Amino Acids
Serum

ASJC Scopus subject areas

  • Virology

Cite this

Joelson, T., Åkerblom, L., Oxelfelt, P., Strandberg, B., Tomenius, K., & Morris, T. J. (1997). Presentation of a foreign peptide on the surface of tomato bushy stunt virus. Journal of General Virology, 78(6), 1213-1217. https://doi.org/10.1099/0022-1317-78-6-1213

Presentation of a foreign peptide on the surface of tomato bushy stunt virus. / Joelson, Thorleif; Åkerblom, Lennart; Oxelfelt, Per; Strandberg, Bror; Tomenius, Karin; Morris, T. Jack.

In: Journal of General Virology, Vol. 78, No. 6, 06.1997, p. 1213-1217.

Research output: Contribution to journalArticle

Joelson, T, Åkerblom, L, Oxelfelt, P, Strandberg, B, Tomenius, K & Morris, TJ 1997, 'Presentation of a foreign peptide on the surface of tomato bushy stunt virus', Journal of General Virology, vol. 78, no. 6, pp. 1213-1217. https://doi.org/10.1099/0022-1317-78-6-1213
Joelson T, Åkerblom L, Oxelfelt P, Strandberg B, Tomenius K, Morris TJ. Presentation of a foreign peptide on the surface of tomato bushy stunt virus. Journal of General Virology. 1997 Jun;78(6):1213-1217. https://doi.org/10.1099/0022-1317-78-6-1213
Joelson, Thorleif ; Åkerblom, Lennart ; Oxelfelt, Per ; Strandberg, Bror ; Tomenius, Karin ; Morris, T. Jack. / Presentation of a foreign peptide on the surface of tomato bushy stunt virus. In: Journal of General Virology. 1997 ; Vol. 78, No. 6. pp. 1213-1217.
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