Presence of CD8+ T cells in the ectocervical mucosa correlates with genital viral shedding in HIV-infected women despite a low prevalence of HIV RNA-expressing cells in the tissue

Anna Gibbs, Taha Hirbod, Qingsheng Li, Karin Bohman, Terry B. Ball, Francis A. Plummer, Rupert Kaul, Joshua Kimani, Kristina Broliden, Annelie Tjernlund

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The female genital tract is a portal of entry for sexual HIV transmission and a possible viral reservoir. In this study, the ectocervical CD8+ T cell distribution was explored in situ and was related to expression of CD3 and HLA-DR and presence of HIV RNA. For this purpose, ectocervical tissue samples and genital secretions were collected from HIV-seropositive (HIV +) Kenyan female sex workers (FSWs) (n = 20), HIV-seronegative (HIV-) FSWs (n = 17), and HIV- lower-risk women (n = 21). Cell markers were assessed by in situ staining and by quantitative PCR. HIV RNA expression in tissue was analyzed by in situ hybridization, and viral shedding was assessed by quantitative PCR. The HIV+FSW group had a higher amount of total cells and CD8+, CD3+, and HLA-DR + cells compared with the HIV-FSWgroup and HIV- lower-risk women. The majority of CD8+ cells were CD3+ T cells, and the numbers of CD8+ cells correlated significantly with plasma and cervical viral load. HIV RNA expression in situ was found in 4 of the 20 HIV+FSW women but did not correlate with cervical or plasma viral load. Thus, the HIV+ women displayed high numbers of CD8 +, CD3+, and HLA-DR+ cells, as well as a limited number of HIV RNA+ cells, in their ectocervical mucosa; hence, this localization cannot be neglected as a potential viral reservoir. The elevated levels of CD8+ T cells may play a role in the immunopathogenesis of HIV in the female genital tract.

Original languageEnglish (US)
Pages (from-to)3947-3957
Number of pages11
JournalJournal of Immunology
Volume192
Issue number8
DOIs
StatePublished - Apr 15 2014

Fingerprint

Virus Shedding
Mucous Membrane
HIV
RNA
T-Lymphocytes
Sex Workers
HLA-DR Antigens
Viral Load
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Presence of CD8+ T cells in the ectocervical mucosa correlates with genital viral shedding in HIV-infected women despite a low prevalence of HIV RNA-expressing cells in the tissue. / Gibbs, Anna; Hirbod, Taha; Li, Qingsheng; Bohman, Karin; Ball, Terry B.; Plummer, Francis A.; Kaul, Rupert; Kimani, Joshua; Broliden, Kristina; Tjernlund, Annelie.

In: Journal of Immunology, Vol. 192, No. 8, 15.04.2014, p. 3947-3957.

Research output: Contribution to journalArticle

Gibbs, Anna ; Hirbod, Taha ; Li, Qingsheng ; Bohman, Karin ; Ball, Terry B. ; Plummer, Francis A. ; Kaul, Rupert ; Kimani, Joshua ; Broliden, Kristina ; Tjernlund, Annelie. / Presence of CD8+ T cells in the ectocervical mucosa correlates with genital viral shedding in HIV-infected women despite a low prevalence of HIV RNA-expressing cells in the tissue. In: Journal of Immunology. 2014 ; Vol. 192, No. 8. pp. 3947-3957.
@article{034360c71bea4f31b91f4f7b8d2f4cdb,
title = "Presence of CD8+ T cells in the ectocervical mucosa correlates with genital viral shedding in HIV-infected women despite a low prevalence of HIV RNA-expressing cells in the tissue",
abstract = "The female genital tract is a portal of entry for sexual HIV transmission and a possible viral reservoir. In this study, the ectocervical CD8+ T cell distribution was explored in situ and was related to expression of CD3 and HLA-DR and presence of HIV RNA. For this purpose, ectocervical tissue samples and genital secretions were collected from HIV-seropositive (HIV +) Kenyan female sex workers (FSWs) (n = 20), HIV-seronegative (HIV-) FSWs (n = 17), and HIV- lower-risk women (n = 21). Cell markers were assessed by in situ staining and by quantitative PCR. HIV RNA expression in tissue was analyzed by in situ hybridization, and viral shedding was assessed by quantitative PCR. The HIV+FSW group had a higher amount of total cells and CD8+, CD3+, and HLA-DR + cells compared with the HIV-FSWgroup and HIV- lower-risk women. The majority of CD8+ cells were CD3+ T cells, and the numbers of CD8+ cells correlated significantly with plasma and cervical viral load. HIV RNA expression in situ was found in 4 of the 20 HIV+FSW women but did not correlate with cervical or plasma viral load. Thus, the HIV+ women displayed high numbers of CD8 +, CD3+, and HLA-DR+ cells, as well as a limited number of HIV RNA+ cells, in their ectocervical mucosa; hence, this localization cannot be neglected as a potential viral reservoir. The elevated levels of CD8+ T cells may play a role in the immunopathogenesis of HIV in the female genital tract.",
author = "Anna Gibbs and Taha Hirbod and Qingsheng Li and Karin Bohman and Ball, {Terry B.} and Plummer, {Francis A.} and Rupert Kaul and Joshua Kimani and Kristina Broliden and Annelie Tjernlund",
year = "2014",
month = "4",
day = "15",
doi = "10.4049/jimmunol.1302826",
language = "English (US)",
volume = "192",
pages = "3947--3957",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - Presence of CD8+ T cells in the ectocervical mucosa correlates with genital viral shedding in HIV-infected women despite a low prevalence of HIV RNA-expressing cells in the tissue

AU - Gibbs, Anna

AU - Hirbod, Taha

AU - Li, Qingsheng

AU - Bohman, Karin

AU - Ball, Terry B.

AU - Plummer, Francis A.

AU - Kaul, Rupert

AU - Kimani, Joshua

AU - Broliden, Kristina

AU - Tjernlund, Annelie

PY - 2014/4/15

Y1 - 2014/4/15

N2 - The female genital tract is a portal of entry for sexual HIV transmission and a possible viral reservoir. In this study, the ectocervical CD8+ T cell distribution was explored in situ and was related to expression of CD3 and HLA-DR and presence of HIV RNA. For this purpose, ectocervical tissue samples and genital secretions were collected from HIV-seropositive (HIV +) Kenyan female sex workers (FSWs) (n = 20), HIV-seronegative (HIV-) FSWs (n = 17), and HIV- lower-risk women (n = 21). Cell markers were assessed by in situ staining and by quantitative PCR. HIV RNA expression in tissue was analyzed by in situ hybridization, and viral shedding was assessed by quantitative PCR. The HIV+FSW group had a higher amount of total cells and CD8+, CD3+, and HLA-DR + cells compared with the HIV-FSWgroup and HIV- lower-risk women. The majority of CD8+ cells were CD3+ T cells, and the numbers of CD8+ cells correlated significantly with plasma and cervical viral load. HIV RNA expression in situ was found in 4 of the 20 HIV+FSW women but did not correlate with cervical or plasma viral load. Thus, the HIV+ women displayed high numbers of CD8 +, CD3+, and HLA-DR+ cells, as well as a limited number of HIV RNA+ cells, in their ectocervical mucosa; hence, this localization cannot be neglected as a potential viral reservoir. The elevated levels of CD8+ T cells may play a role in the immunopathogenesis of HIV in the female genital tract.

AB - The female genital tract is a portal of entry for sexual HIV transmission and a possible viral reservoir. In this study, the ectocervical CD8+ T cell distribution was explored in situ and was related to expression of CD3 and HLA-DR and presence of HIV RNA. For this purpose, ectocervical tissue samples and genital secretions were collected from HIV-seropositive (HIV +) Kenyan female sex workers (FSWs) (n = 20), HIV-seronegative (HIV-) FSWs (n = 17), and HIV- lower-risk women (n = 21). Cell markers were assessed by in situ staining and by quantitative PCR. HIV RNA expression in tissue was analyzed by in situ hybridization, and viral shedding was assessed by quantitative PCR. The HIV+FSW group had a higher amount of total cells and CD8+, CD3+, and HLA-DR + cells compared with the HIV-FSWgroup and HIV- lower-risk women. The majority of CD8+ cells were CD3+ T cells, and the numbers of CD8+ cells correlated significantly with plasma and cervical viral load. HIV RNA expression in situ was found in 4 of the 20 HIV+FSW women but did not correlate with cervical or plasma viral load. Thus, the HIV+ women displayed high numbers of CD8 +, CD3+, and HLA-DR+ cells, as well as a limited number of HIV RNA+ cells, in their ectocervical mucosa; hence, this localization cannot be neglected as a potential viral reservoir. The elevated levels of CD8+ T cells may play a role in the immunopathogenesis of HIV in the female genital tract.

UR - http://www.scopus.com/inward/record.url?scp=84898632823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898632823&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.1302826

DO - 10.4049/jimmunol.1302826

M3 - Article

C2 - 24639358

AN - SCOPUS:84898632823

VL - 192

SP - 3947

EP - 3957

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -