Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers

Garrett A. Soukup, Ronald L. Cerny, Ronald Cerny

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalBioconjugate Chemistry
Volume6
Issue number1
DOIs
StatePublished - Jan 1 1995

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Oligonucleotides
Biotin
Dithiothreitol
Disulfides
Amines
Purification
Esters
N-hydroxysulfosuccinimide
streptavidin-agarose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers. / Soukup, Garrett A.; Cerny, Ronald L.; Cerny, Ronald.

In: Bioconjugate Chemistry, Vol. 6, No. 1, 01.01.1995, p. 135-138.

Research output: Contribution to journalArticle

Soukup, Garrett A. ; Cerny, Ronald L. ; Cerny, Ronald. / Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers. In: Bioconjugate Chemistry. 1995 ; Vol. 6, No. 1. pp. 135-138.
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