Abstract
A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 135-138 |
| Number of pages | 4 |
| Journal | Bioconjugate Chemistry |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1995 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
Cite this
Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers. / Soukup, Garrett A.; Cerny, Ronald L.; Cerny, Ronald.
In: Bioconjugate Chemistry, Vol. 6, No. 1, 01.01.1995, p. 135-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers
AU - Soukup, Garrett A.
AU - Cerny, Ronald L.
AU - Cerny, Ronald
PY - 1995/1/1
Y1 - 1995/1/1
N2 - A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.
AB - A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029206756&partnerID=8YFLogxK
U2 - 10.1021/bc00031a017
DO - 10.1021/bc00031a017
M3 - Article
C2 - 7711100
AN - SCOPUS:0029206756
VL - 6
SP - 135
EP - 138
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 1
ER -