Preparation of high-capacity supports containing protein G immobilized to porous silica

Abby J. Jackson, Elizabeth M. Karle, David S Hage

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100Å pore size silica, followed by 33mg/g for 50Å silica and 9.3-24mg/g for 300-4000Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m2 being obtained for 4000Å silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100Å silica.

Original languageEnglish (US)
Pages (from-to)235-237
Number of pages3
JournalAnalytical Biochemistry
Volume406
Issue number2
DOIs
StatePublished - Nov 1 2010

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Immobilized Proteins
Silicon Dioxide
Proteins
Pore size
Immunoglobulin G
Membrane Proteins
Rabbits

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Preparation of high-capacity supports containing protein G immobilized to porous silica. / Jackson, Abby J.; Karle, Elizabeth M.; Hage, David S.

In: Analytical Biochemistry, Vol. 406, No. 2, 01.11.2010, p. 235-237.

Research output: Contribution to journalArticle

Jackson, Abby J. ; Karle, Elizabeth M. ; Hage, David S. / Preparation of high-capacity supports containing protein G immobilized to porous silica. In: Analytical Biochemistry. 2010 ; Vol. 406, No. 2. pp. 235-237.
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