Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing.

Y. Xiao, Rebecca Y. Lai, Kevin W. Plaxco

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

Recent years have seen the development of a number of reagentless, electrochemical sensors based on the target-induced folding or unfolding of electrode-bound oligonucleotides, with examples reported to date, including sensors for the detection of specific nucleic acids, proteins, small molecules and inorganic ions. These devices, which are often termed electrochemical DNA (E-DNA) and E-AB (electrochemical, aptamer-based) sensors, are comprised of an oligonucleotide probe modified with a redox reporter (in this protocol methylene blue) at one terminus and attached to a gold electrode via a thiol-gold bond at the other. Binding of an analyte to the oligonucleotide probe changes its structure and dynamics, which, in turn, influences the efficiency of electron transfer to the interrogating electrode. This class of sensors perform well even when challenged directly with blood serum, soil and other complex, multicomponent sample matrices. This protocol describes the fabrication of E-DNA and E-AB sensors. The protocol can be completed in 12 h.

Original languageEnglish (US)
Pages (from-to)2875-2880
Number of pages6
JournalNature protocols
Volume2
Issue number11
DOIs
StatePublished - 2007

Fingerprint

Nucleotide Aptamers
Oligonucleotides
Oxidation-Reduction
Electrodes
Oligonucleotide Probes
Gold
Sensors
Methylene Blue
Sulfhydryl Compounds
Electrochemical sensors
Nucleic Acids
Soil
Electrons
Ions
Equipment and Supplies
Blood
Serum
Soils
Fabrication
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing. / Xiao, Y.; Lai, Rebecca Y.; Plaxco, Kevin W.

In: Nature protocols, Vol. 2, No. 11, 2007, p. 2875-2880.

Research output: Contribution to journalArticle

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