Preparation of DNA complexes with diblock copolymers of poly[N(2-hydroxypropyl)methacrylamide] and polycations

David Oupicky, Č Koňák, K. Ulbrich

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study investigated the effects of the speed of component mixing, total concentration of polymers and ionic strength of solvents on formation of interpolyelectrolyte complexes (IPECs) of calf thymus DNA with diblock copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and 2-(trimethylammonio)ethyl methacrylate (TMAEMCl). The diblock copolymer consisting of a poly(HPMA) block (A) and poly(TMAEMCl) polycation block (B) with pendant quaternary ammonium groups and with molecular weight of A block higher than that of B was used in the study. Poly(TMAEMCl) polycation were used for comparison. Static and dynamic light scattering methods were used for characterisation of complexes. The apparent mass-average molecular weights, M(w)a, and hydrodynamic radii, R(H)a, of slightly overcompensated IPECs (φ = [cation units]/[DNA negative charges] = 1.1) prepared by fast mixing of components were found to be only slightly smaller than those prepared by slow mixing. Both M(w)a and R(H)a of IPECs prepared under stoichiometric conditions (φ = 1) decreased with decreasing total polymer concentration, c(T), approaching M(w) and R(H) of single DNA complexes which are the most suitable ones for DNA uptake into cells. The presence of NaCl in solutions resulted in a significant increase in M(w)a and R(H)a of IPECS.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalMaterials Science and Engineering C
Volume7
Issue number1
DOIs
StatePublished - May 1 1999

Fingerprint

Block copolymers
copolymers
DNA
Methacrylates
deoxyribonucleic acid
preparation
Polymers
Molecular weight
molecular weight
Dynamic light scattering
Ionic strength
Ammonium Compounds
Thymus
calves
Cations
polymers
Hydrodynamics
light scattering
Positive ions
hydrodynamics

Keywords

  • Calf thymus DNA
  • Diblock copolymers
  • Interpolyelectrolyte complexes
  • Light scattering methods
  • Polycations
  • Self-assembly

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Preparation of DNA complexes with diblock copolymers of poly[N(2-hydroxypropyl)methacrylamide] and polycations. / Oupicky, David; Koňák, Č; Ulbrich, K.

In: Materials Science and Engineering C, Vol. 7, No. 1, 01.05.1999, p. 59-65.

Research output: Contribution to journalArticle

@article{b9e27f0b43294c27839d5be2f013df5e,
title = "Preparation of DNA complexes with diblock copolymers of poly[N(2-hydroxypropyl)methacrylamide] and polycations",
abstract = "This study investigated the effects of the speed of component mixing, total concentration of polymers and ionic strength of solvents on formation of interpolyelectrolyte complexes (IPECs) of calf thymus DNA with diblock copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and 2-(trimethylammonio)ethyl methacrylate (TMAEMCl). The diblock copolymer consisting of a poly(HPMA) block (A) and poly(TMAEMCl) polycation block (B) with pendant quaternary ammonium groups and with molecular weight of A block higher than that of B was used in the study. Poly(TMAEMCl) polycation were used for comparison. Static and dynamic light scattering methods were used for characterisation of complexes. The apparent mass-average molecular weights, M(w)a, and hydrodynamic radii, R(H)a, of slightly overcompensated IPECs (φ = [cation units]/[DNA negative charges] = 1.1) prepared by fast mixing of components were found to be only slightly smaller than those prepared by slow mixing. Both M(w)a and R(H)a of IPECs prepared under stoichiometric conditions (φ = 1) decreased with decreasing total polymer concentration, c(T), approaching M(w) and R(H) of single DNA complexes which are the most suitable ones for DNA uptake into cells. The presence of NaCl in solutions resulted in a significant increase in M(w)a and R(H)a of IPECS.",
keywords = "Calf thymus DNA, Diblock copolymers, Interpolyelectrolyte complexes, Light scattering methods, Polycations, Self-assembly",
author = "David Oupicky and Č Koň{\'a}k and K. Ulbrich",
year = "1999",
month = "5",
day = "1",
doi = "10.1016/S0928-4931(99)00003-X",
language = "English (US)",
volume = "7",
pages = "59--65",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Preparation of DNA complexes with diblock copolymers of poly[N(2-hydroxypropyl)methacrylamide] and polycations

AU - Oupicky, David

AU - Koňák, Č

AU - Ulbrich, K.

PY - 1999/5/1

Y1 - 1999/5/1

N2 - This study investigated the effects of the speed of component mixing, total concentration of polymers and ionic strength of solvents on formation of interpolyelectrolyte complexes (IPECs) of calf thymus DNA with diblock copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and 2-(trimethylammonio)ethyl methacrylate (TMAEMCl). The diblock copolymer consisting of a poly(HPMA) block (A) and poly(TMAEMCl) polycation block (B) with pendant quaternary ammonium groups and with molecular weight of A block higher than that of B was used in the study. Poly(TMAEMCl) polycation were used for comparison. Static and dynamic light scattering methods were used for characterisation of complexes. The apparent mass-average molecular weights, M(w)a, and hydrodynamic radii, R(H)a, of slightly overcompensated IPECs (φ = [cation units]/[DNA negative charges] = 1.1) prepared by fast mixing of components were found to be only slightly smaller than those prepared by slow mixing. Both M(w)a and R(H)a of IPECs prepared under stoichiometric conditions (φ = 1) decreased with decreasing total polymer concentration, c(T), approaching M(w) and R(H) of single DNA complexes which are the most suitable ones for DNA uptake into cells. The presence of NaCl in solutions resulted in a significant increase in M(w)a and R(H)a of IPECS.

AB - This study investigated the effects of the speed of component mixing, total concentration of polymers and ionic strength of solvents on formation of interpolyelectrolyte complexes (IPECs) of calf thymus DNA with diblock copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and 2-(trimethylammonio)ethyl methacrylate (TMAEMCl). The diblock copolymer consisting of a poly(HPMA) block (A) and poly(TMAEMCl) polycation block (B) with pendant quaternary ammonium groups and with molecular weight of A block higher than that of B was used in the study. Poly(TMAEMCl) polycation were used for comparison. Static and dynamic light scattering methods were used for characterisation of complexes. The apparent mass-average molecular weights, M(w)a, and hydrodynamic radii, R(H)a, of slightly overcompensated IPECs (φ = [cation units]/[DNA negative charges] = 1.1) prepared by fast mixing of components were found to be only slightly smaller than those prepared by slow mixing. Both M(w)a and R(H)a of IPECs prepared under stoichiometric conditions (φ = 1) decreased with decreasing total polymer concentration, c(T), approaching M(w) and R(H) of single DNA complexes which are the most suitable ones for DNA uptake into cells. The presence of NaCl in solutions resulted in a significant increase in M(w)a and R(H)a of IPECS.

KW - Calf thymus DNA

KW - Diblock copolymers

KW - Interpolyelectrolyte complexes

KW - Light scattering methods

KW - Polycations

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=0344500689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344500689&partnerID=8YFLogxK

U2 - 10.1016/S0928-4931(99)00003-X

DO - 10.1016/S0928-4931(99)00003-X

M3 - Article

VL - 7

SP - 59

EP - 65

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

IS - 1

ER -