Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering

Anuradha Subramanian, David Vu, Gustavo Larsen, Hsin Yi Lin

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Fibrous materials have morphological similarities to natural cartilage extracellular matrix and have been considered as candidate for bone tissue engineering scaffolds. In this study, we have evaluated a novel electrospun chitosan mat composed of oriented sub-micron fibers for its tensile property and biocompatibility with chondrocytes (cell attachment, proliferation and viability). Scanning electronic microscope images showed the fibers in the electrospun chitosan mats were indeed aligned and there was a slight cross-linking between the parent fibers. The electrospun mats have significantly higher elastic modulus (2.25 MPa) than the cast films (1.19 MPa). Viability of cells on the electrospun mat was 69% of the cells on tissue-culture polystyrene (TCP control) after three days in culture, which was slightly higher than that on the cast films (63% of the TCP control). Cells on the electrospun mat grew slowly the first week but the growth rate increased after that. By day 10, cell number on the electrospun mat was almost 82% that of TCP control, which was higher than that of cast films (56% of TCP). The electrospun chitosan mats have a higher Young's modulus (P<0.01) than cast films and provide good chondrocyte biocompatibility. The electrospun chitosan mats, thus, have the potential to be further processed into three-dimensional scaffolds for cartilage tissue repair.

Original languageEnglish (US)
Pages (from-to)861-873
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume16
Issue number7
DOIs
StatePublished - Jul 1 2005

Fingerprint

Bioelectric potentials
Chitosan
Cartilage
Tissue Engineering
Polyethylene oxides
Tissue engineering
Tissue Scaffolds
Fibers
Elastic Modulus
Chondrocytes
Biocompatibility
Cell Survival
Elastic moduli
Tissue culture
Polystyrenes
Scaffolds (biology)
Tensile properties
Scaffolds
Extracellular Matrix
Bone

Keywords

  • Cartilage repair
  • Chitosan nanofiber
  • Chondrocyte
  • Electrospinning
  • Tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

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title = "Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering",
abstract = "Fibrous materials have morphological similarities to natural cartilage extracellular matrix and have been considered as candidate for bone tissue engineering scaffolds. In this study, we have evaluated a novel electrospun chitosan mat composed of oriented sub-micron fibers for its tensile property and biocompatibility with chondrocytes (cell attachment, proliferation and viability). Scanning electronic microscope images showed the fibers in the electrospun chitosan mats were indeed aligned and there was a slight cross-linking between the parent fibers. The electrospun mats have significantly higher elastic modulus (2.25 MPa) than the cast films (1.19 MPa). Viability of cells on the electrospun mat was 69{\%} of the cells on tissue-culture polystyrene (TCP control) after three days in culture, which was slightly higher than that on the cast films (63{\%} of the TCP control). Cells on the electrospun mat grew slowly the first week but the growth rate increased after that. By day 10, cell number on the electrospun mat was almost 82{\%} that of TCP control, which was higher than that of cast films (56{\%} of TCP). The electrospun chitosan mats have a higher Young's modulus (P<0.01) than cast films and provide good chondrocyte biocompatibility. The electrospun chitosan mats, thus, have the potential to be further processed into three-dimensional scaffolds for cartilage tissue repair.",
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