Preliminary X-ray diffraction studies of the transcriptional inhibitory antibody Fab41.4

J. Mazlo, R. L. Stanfield, I. A. Wilson, Steven Heye Hinrichs, J. J. Stezowski

Research output: Contribution to journalArticle

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Abstract

The binding of transcription factor ATF-1 to DNA contributes to gene expression and regulation of cell growth. Antibody Mab41.4, raised against ATF-1, and its derivatives Fab41.4 and scFv41.4 inhibit specific DNA binding in vitro and induce apoptotic death of tumor cells in vivo. Structural studies of Fab41.4 were performed to gain insight into the mechanism of action of this potentially therapeutic antibody. The optimal conditions for crystallization of Fab41.4 were determined. Crystals were needle-like in appearance, displayed C2 space-group symmetry and diffracted to a resolution of 1.6 Å. The unit-cell parameters were determined to be a = 186.64, b = 40.22, c = 55.58 Å, α = γ = 90, β = 96.93°. The data set was 97.7% complete. Molecular replacement was performed, resulting in an R value of 44.6%.

Original languageEnglish (US)
Pages (from-to)462-464
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number3
DOIs
StatePublished - Mar 21 2001

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F-22 aircraft
gene expression
antibodies
Gene expression
X-Ray Diffraction
deoxyribonucleic acid
Activating Transcription Factors
X ray diffraction
Antibodies
DNA
Gene Expression Regulation
Cell growth
Crystallization
diffraction
death
needles
Needles
Tumors
Cell Death
x rays

ASJC Scopus subject areas

  • Structural Biology

Cite this

Preliminary X-ray diffraction studies of the transcriptional inhibitory antibody Fab41.4. / Mazlo, J.; Stanfield, R. L.; Wilson, I. A.; Hinrichs, Steven Heye; Stezowski, J. J.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 3, 21.03.2001, p. 462-464.

Research output: Contribution to journalArticle

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