Preliminary studies on the use of pertussis toxin for the modulation of intravaginal SIV transmission in rhesus macaques

Kristina Ortiz, Rebecca S. Sampathkumar, Aftab A. Ansari, Siddappa Nagadenahalli Byrareddy

Research output: Contribution to journalArticle

Abstract

Background: Pertussis toxin (PTX) blocks GPCR signaling resulting in the inhibition of chemotaxis/cell adhesion. It was reasoned that inhibition of cell trafficking may be an approach to prevent HIV/SIV transmission. Methods: In this study, PTX in HEC gel was applied to the vaginal wall of monkeys that were then challenged intravaginally with SIVmac251. Results: Results of these studies showed that 2 of 4 animals were resistant to infection. Furthermore, infection was correlated with a marked increase in the plasma and cervicovaginal lavage levels of select chemokines and cytokines. Conclusions: Results from this preliminary feasibility study dictate that further studies that include a larger number of animals are required to optimize this protocol and establish the efficacy of this approach. In addition, such future studies will provide important information on the role of specific chemokines that play a role in lymphocyte trafficking within the genital tract and serve as additional therapeutic targets.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalJournal of medical primatology
Volume46
Issue number6
DOIs
Publication statusPublished - Dec 2017

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Keywords

  • G-protein–coupled receptors
  • SIVmac251
  • pertussis toxin
  • rhesus macaques
  • vaginal transmission

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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